The mature larvae of three species of Hoplitis, namely Hoplitis anceyi (Pérez), Hoplitis anthodemnion Michener, and Hoplitis nitidula (Morawitz), are described and compared with the other known larvae in the genus. The morphology of the spiracles, antennal discs, and antennal papillae, the absence of a seta on the mandibular basis, and the shape of the vertex permit the characterization of each species. Furthermore, H. anthodemnion has the integument covered by setiform spicules. Nevertheless, there is no clear separation between the three species studied and those previously known, and larval morphology does not support the consideration of Anthocopa and Hoplitis as separate genera.