A 3-yr study was initiated in 1982 to determine the effects of herbicides and crop rotations on large crabgrass [Digitaria sanguinalis (L.) Scop. # DIGSA] and broadleaf signalgrass [Brachiaria platyphylla (Griseb.) Nash # BRAPP] population dynamics. Regardless of the crop rotation sequence, broadleaf signalgrass immediately became the predominant weed where standard herbicide programs were used. Large crabgrass became the predominant species after two growing seasons if no herbicides were applied. Domination by large crabgrass appeared to be due to greater seed production. The domination by broadleaf signalgrass in plots treated with herbicides was attributed to its tolerance to the primary grass herbicide alachlor [2-chloro-N-(2,6-diethylphenyl)-N-methoxymethyl)acetamide]. Broadleaf signalgrass emergence from soil treated with 2.2 kg ai/ha was not statistically different from that in untreated soil, while large crabgrass and fall panicum [Panicum dichotomiflorum (L.) Michx. # PANDI] emergence was significantly reduced at the same rate.

Field experiments determined the potential interaction of insecticides and propanil [N-(3,4-dichlorophenyl) propanamide] in drill-seeded rice (Oryza sativa L. ‘Bond’). The carbamate insecticides carbaryl (1-naphthylmethylcarbamate) and methomyl {S-methyl N-[(methylcarbamoyl) oxy] thioacetimidate} interacted more severely with propanil than the organophosphate methyl parathion [O,O-dimethyl O-(p-nitrophenyl) phosphorothioate] in emulsifiable or encapsulated formulations. Carbaryl and methomyl injured rice vegetatively when applied from 7 days before to 4 days after propanil. Carbaryl was the only insecticide that interacted with propanil to reduce rice grain yield. Insecticides interacted adversely with propanil on rice grain yield when they were applied 2 days before propanil treatment. Insecticide interactions with propanil from most to least phytotoxic to rice plants for all times of application were: carbaryl > methomyl > emulsifiable methyl parathion > encapsulated methyl parathion.

The effects of the dimethylamine salt of dicamba (3,6-dichloro-2-methoxybenzoic acid) and the dimethylamine salt of 2,4-D [(2,4-dichlorophenoxy)acetic acid] on fieldbeans (Phaseolus vulgaris L. ‘Great Northern Valley’) were studied in order to assess the potential hazards of using these herbicides in areas adjoining fieldbean production. Dicamba and 2,4-D were applied to fieldbeans at three different rates (1.1, 11.2, and 112.5 g ai/ha) and four different growth stages (preemergence, second trifoliolate leaf, early bloom, and early pod). Application of 2,4-D preemergence or in the second trifoliolate leaf stage of growth did not reduce seed yield, delay maturity, or reduce germination of seed obtained from treated plants. Dicamba or 2,4-D applied at 112.5 g/ha to fieldbeans in the early bloom or early pod stages of growth consistently reduced seed yield, delayed maturity, and reduced germination percentage. Fieldbeans exhibited a greater overall sensitivity to dicamba than to 2,4-D.

Carbon-14-labeled glyphosate [N-(phosphonomethyl)glycine] was foliarly applied to ligustrum (Ligustrum japonicum Thunb.) and blue pacific juniper (Juniperus conferta Parl. ‘Blue Pacific’) at the following growth stages: cold acclimation, winter dormancy, budbreak, shoot elongation, and termination of the first flush of growth (shoot termination). At shoot elongation juniper plants absorbed 2% of applied 14C by 14 days after treatment (DAT). Applications at other growth stages resulted in no significant absorption of 14C by junipers. Within 7 DAT the amounts of radioactivity absorbed by ligustrum were significant and depended upon growth stage in the following order: budbreak < shoot termination < shoot elongation. Absorption by overwintered leaves occurred at budbreak but not at elongation or termination. Transport of absorbed 14C in ligustrum was primarily acropetal and occurred only in budbreak and flowering treatments. Differences in tolerance to glyphosate between juniper and ligustrum appear to be related to differential absorption. Seasonal differences in ligustrum tolerance also appear to be associated with differences in absorption. Although growth stage affected transport in ligustrum, differential transport does not appear to play a major role in seasonal influences on long-term glyphosate phytotoxicity.

Paraquat (1,1′-dimethyl-4,4′-bipyridinium ion) was applied to peanuts (Arachis hypogaea L. ‘Florunner’) for Texas panicum (Panicum texanum Buckl. # PANTE) control at 0.07, 0.14, and 0.28 kg ai/ha under five application schedules; a single application at 0, 1, 3, or 5 weeks after ground cracking, or multiple applications at 1 + 5 weeks or 1 + 3 + 5 weeks after ground cracking. Peanuts were planted at two different times (normal and late). A herbicide application at the third week (normal planting), or the third or fifth week after ground cracking (late planting) provided acceptable Texas panicum control. Control generally increased with rate. However, increasing paraquat rates with single applications on late-planted peanuts or multiple applications to normal- and late-planted peanuts tended to reduce yield and grade. A single application of 0.07 or 0.14 kg/ha applied the third week after ground cracking consistently provided the best balance between Texas panicum control and crop safety.

Field research was conducted in 1982 and 1983 to characterize the persistence of fluridone {1-methyl-3-phenyl-5-[3-(trifluoromethyl)phenyl]-4(1H)pyridinone} in five Georgia soils. Fluridone persisted less than 365 days in all soils, with shorter persistence upon reapplication in 1983 which indicated the potential for enhanced microbial degradation. A significantly higher rate of loss in 1983 compared to 1982 was recorded in the Greenville sandy clay and Dothan loamy sand soils. A higher rate of loss was recorded for the 1.7 kg ai/ha than the 0.6 kg/ha treatment in the Bradson clay loam and Rome gravelly clay loam soils. No grain sorghum [Sorghum bicolor (L.) Moench. ‘BR 64’) injury was observed in a field bio assay planted in the spring of 1984. Herbicide leaching did not appear to be an important method of loss.

Velvetleaf (Abutilon theophrasti Medik. # ABUTH) seeds were bioassayed on 241 microbial isolates to assess their antimicrobial activity. Seeds placed on agar plates inoculated with test microorganisms released a diffusible substance(s) that inhibited the growth of 117 of 202 (58%) bacteria and all of the fungi tested. Antimicrobial activity of the seeds appeared to be nonselective as the extent of inhibition was not related to type of microorganism nor their origin. Hard, water-impermeable seeds had greater inhibitory activity than imbibed (soft) seeds. The intensity of inhibition was affected by prior leaching of seeds with various solvents and by the stage of seed development. Chemical analysis of diffusion zones from agar plates and seed leachates revealed the presence of phenolic compounds. The presence of antimicrobial substances in velvetleaf seeds may contribute to the persistence of viable seeds in soil by inhibiting potential seed-deteriorating microorganisms.

Sethoxydim {2-[1-(ethoxyimino)butyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-one} is used to control weedy grasses in red fescue (Festuca rubra L. # FESRU and F. rubra ssp. commutata), an important seed crop in Oregon. The sethoxydim rate required to reduce growth rate of 11 cultivars of red fescue and four cultivars of bentgrass (Agrostis tenuis Sibth. # AGSTE and A. stolonifera L. var. palustris # AGSST), a common contaminant in red fescue fields, by 50% (GR50) was determined in greenhouse and laboratory experiments. Field experiments compared only red fescue cv. ‘Pennlawn’ and bentgrass cv. ‘Penncross'. The GR50 values for Pennlawn red fescue in the field, the greenhouse, and the laboratory were 30 kg ai/ha, 15 kg/ha, and 5300 μM, respectively. Penncross bentgrass was approximately 400, 1400, and 12 900 times more susceptible than Pennlawn red fescue in the field, in the greenhouse, and in laboratory experiments, respectively. Plants were at different growth stages in the different experiments. The xylene and surfactant used as a solvent for the technical grade sethoxydim was as toxic to red fescue without sethoxydim as when sethoxydim was included. Cultivars of red fescue differed in their response to sethoxydim, but cultivars of bentgrass did not. The GR50's of other species tested in the greenhouse were >4 kg/ha for annual bluegrass (Poa annua L. # POAAN), 0.22 kg/ha for tall fescue (F. arundinacea Schreb. # FESAR), 0.37 kg/ha for downy brome (Bromus tectorum L. # BROTE), and 0.017 kg/ha for Italian ryegrass (Lolium multiflorum Lam. # LOLMU).

Five varieties of 3-month-old citrus rootstocks were grown for 1 month in nutrient solution containing 0, 10-3, 10-4, or 10-5 M of norflurazon [4-chloro-5-(methylamino)-2-(3-(trifluoromethyl)phenyl)-3 (2H)-pyridazinone] to assess the effect of this herbicide on growth and chlorophyll levels. The uptake, translocation, and metabolism of root-applied 14C-norflurazon was also examined in these rootstocks. Norflurazon treatment reduced chlorophyll level and lowered overall growth in sour orange (Citrus aurantium), Swingle (Poncirus trifoliata × C. paradisi), Milam (C. jambhiri), and Cleopatra mandarin (C. reshni) rootstocks. Herbicide treatments had little or no effect on chlorophyll levels or growth in Carrizo (C. sinensis × P. trifoliata). In Cleopatra, unlike in other rootstocks, leaf chlorosis was evident only along the midrib and major veins. Herbicide treatment reduced leaf flush (leaf production) in Swingle but not in other rootstocks. By the end of a 4-week treatment period, total absorption of 14C-norflurazon was greater in Swingle than in the other rootstocks. 14C-norflurazon detected in the roots (excluding taproot) of Swingle was greater than in the other rootstocks. The amount of 14C-norflurazon translocation to the foliage was limited in all rootstocks. Stem and taproot also contained only a small amount of 14C-norflurazon. Leaf extracts of all five rootstocks contained no parent compound, but up to 90% of recovered 14C was identified as 14C-norflurazon in root extracts. Differences in the uptake, translocation, and metabolism of norflurazon among citrus rootstocks were small and did not appear to explain their differential sensitivity to this herbicide.

Evidence has shown that fluometuron {N,N-dimethyl-N′-[3-(trifluoromethyl)phenyl]urea} persists beyond the end of the growing season when used in continuous cotton (Gossypium hirsutum L.) production. Samples were taken from three soils following cotton production in 1980, 1981, and 1982. All three soils had been in production under the same herbicide use regime, fluometuron preemergence followed by fluometuron plus MSMA (monosodium methanearsonate), since either 1976 or 1977. The fluometuron remaining in each soil was quantified using a greenhouse bioassay and a chemical extraction technique followed by high-performance liquid chromatography determinations. The fluometuron concentrations determined by bioassay and chemical extraction methods had partial correlation coefficients of 0.62, 0.91, and 0.72 for a Sharkey silty clay, a Dundee silt loam, and a Loring silt loam, respectively. Predictive equations were determined for each soil to relate chemical extraction findings to plant response. Bioassay analysis indicated nearly 2 ppmw of fluometuron in the Sharkey silty clay in October 1980, with 1 ppmw in the Dundee silt loam, and approximately 0.27 ppmw in the Loring silt loam with annual application rates of 4.0, 2.9, and 3.5 kg/ha, respectively. Fluometuron concentrations as determined by chemical analysis were 0.83, 0.34, and 0.14 ppmw, respectively. Fluometuron concentrations declined over the winter in all three soils. Samples taken in March of 1981, 1982, and 1983 showed little difference in carryover levels in the Sharkey silty clay but more yearly variation in the other two soils. Fluometuron was found in all three soils to depths of 60 cm, but more than 55% of the fluometuron was found in the upper 15 cm of each soil. A controlled laboratory study conducted with the three soils showed that both cold and dry conditions reduced fluometuron dissipation rates. In the laboratory under conditions favorable for dissipation, fluometuron had a half-life of 26 days in the Dundee silt loam, 43 days in the Loring silt loam, and 73 days in the Sharkey silty clay. In the field, dissipation was very rapid in the Loring silt loam compared to the Dundee silt loam and the Sharkey silty clay.

Multiple applications of several preemergence herbicides were evaluated for weed control and for phytotoxicity to gladiolus (Gladiolus X hortulanus L.) grown from cormels in 1984 and 1985. Oryzalin [4-(dipropylamino)-3,5-dinitrobenzenesulfonamide] consistently provided acceptable control of southern crabgrass [Digitaria ciliaris (Retz.) Koel. # DIGSP], goosegrass [Eleusine indica (L.) Gaertn. # ELEIN], and smooth pigweed (Amaranthus hybridus L. # AMACH) and had minimal effect on gladiolus plant growth even after four applications. Pronamide [3,5-dichloro (N-1,1-dimethyl-2-propynyl)benzamide], alachlor [2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide], and metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide] provided erratic weed control and reduced yields of gladiolus corms.

The response of corn (Zea mays L. ‘Sokota TS 46’), flax (Linum usitatissimum L. ‘Culbert 79’), grain sorghum [Sorghum bicolor (L.) Merr. ‘Sokota 466’), soybeans [Glycine max (L.) Merr. ‘Corsoy 79’], and sunflowers (Helianthus annuus L. ‘Sokata 4000’) to soil residues 12 and 24 months after application of 17, 34, and 68 g ai/ha chlorsulfuron {2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl) amino] carbonyl] benzenesulfonamide} was determined at two locations, Redfield and Watertown, in eastern South Dakota. All crops at Redfield were injured significantly at 17 g/ha, 12 months after application as determined by plant dry weight and visual evaluations. Corn and sorghum were the most susceptible while flax was the least susceptible. Injury at Watertown was significantly less than at Redfield. Differences in carryover were related to a lower soil pH at Watertown.

Butylate [S-ethyl bis(2-methylpropyl)carbamothioate] persistence was compared in three soils that were treated annually with four thiocarbamate herbicides in 1981 and 1982. Butylate degraded faster in soils treated two consecutive years with butylate + dichlormid (2,2-dichloro-N,N-di-2-prophenylacetamide) than in soils treated for 2 yr with EPTC (S-ethyl dipropyl carbamothioate) + dichlormid, cycloate (S-ethyl cyclohexylethylcarbamothioate), vernolate (S-propyl dipropylcarbamothioate) + dichlormid, or from previously untreated soils. Butylate + dichlormid applications in 1981 and 1982 enhanced butylate degradation in 1983 at all locations and reduced weed control at two locations. Previously untreated soil or soil previously treated with two annual applications of butylate + dichlormid was treated with 6 ppmw butylate, incubated for 14 days, analyzed for residual butylate, and bioassayed for herbicide activity. Bioassay indicated higher herbicidal activity than was predicted by the residual butylate concentration. A herbicidally active metabolite may be produced during the soil degradation of butylate.

Greenhouse and laboratory studies were conducted to determine the allelopathic potential of two sweet potato [Ipomoea batatas L. (Lam.)] cultivars, ‘Regal’ and ‘SC 1149-19’. Yellow nutsedge (Cyperus esculentus L. # CYPES) and alfalfa (Medicago sativa L.) plants grown in soil from sweet potato field plots accumulated less dry matter than plants grown in soil from adjacent weedy plots. Growth of yellow nutsedge and alfalfa plants was also reduced when grown in a potting medium containing decomposing sweet potato plants in comparison to plants grown in potting medium alone. When the sweet potato potting medium mixture was incubated at 25 C and tested weekly using an alfalfa growth bioassay, inhibition was high initially but decreased over time and was not observed after a 12-week incubation. Aqueous methanol (50%)-soluble extracts of sweet potato periderm were inhibitory to yellow nutsedge root growth in vermiculite and alfalfa seed germination on filter paper. Regal extracts were inhibitory to yellow nutsedge root growth at a concentration of 2.5 mg periderm extracted/ml, but SC 1149-19 extract was inhibitory only at a concentration eight times higher. Similar differences between cultivars were observed with the alfalfa germination bioassay. Preliminary separation of the Regal periderm extract by paper chromatography indicated the presence of phenolic compounds with a wide range of polarities. Several of these UV-visible compounds were inhibitory to alfalfa germination.

Greenhouse and field experiments were conducted to evaluate the efficacy of chlorsulfuron {2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino] carbonyl] benzenesulfonamide} on Russian thistle (Salsola iberica Sennen and Pau # SASKR) in a spring wheat (Triticum aestivum L.) summer-fallow rotation. In the greenhouse, when chlorsulfuron was applied to Russian thistle at three stages of growth, shoot dry weight was reduced similarly with either 4.4 g ai/ha plus surfactant or 140 g ai/ha plus surfactant. In the field, postharvest control (1981) of Russian thistle with 17.5 to 140 g/ha of chlorsulfuron ranged from 35 to 60% without surfactant and from 76 to 93% with surfactant. During the summer-fallow year (1982), Russian thistle treated in the preflowering stage of growth was not effectively controlled with 17.5 or 35 g/ha. In contrast, residual control from postharvest treatments 1 yr after application ranged from 90 to 100%. Chlorsulfuron applied in the crop (1983) controlled Russian thistle throughout the growing season. All treatments of chlorsulfuron applied in 1981, 1982, and 1983 increased wheat yield at least 75%.

Experiments were conducted at Urbana, IL, in 1983 and 1984 to determine the effect of adjuvants, adjuvant rate, and carrier volume on postemergence weed control with bentazon [3-(1-methylethyl)-(1H)-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide] and sethoxydim {2-[1-(ethoxyimino)butyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-one} in soybeans [Glycine max (L.) Merr. ‘Williams’]. Little difference was observed between a petroleum oil:emulsifier blend (83:17, v/v) (POC) and a soybean oil: emulsifier blend (85:15, v/v) (SBOC) in enhancing control of velvetleaf (Abutilon theophrasti Medik. # ABUTH) with 0.6 or 1.1 kg ai/ha bentazon. Application of bentazon in a carrier volume of 94 L/ha provided velvetleaf control equivalent to that applied in 187 L/ha. Increasing the adjuvant rate from 2.3 to 11.7 L/ha increased visible soybean injury but had no effect on velvetleaf control with bentazon. Control of giant foxtail (Setaria faberi Herrm. # SETFA) with 0.1 kg ai/ha sethoxydim was enhanced more by POC than by SBOC. Phytotoxicity of sethoxydim was not altered by changes in carrier volume or adjuvant rate under conditions of adequate soil moisture in 1983. Under limiting soil moisture in 1984, giant foxtail control with sethoxydim increased slightly when the adjuvant rate was increased from 4.6 to 11.7 L/ha, and carrier volume was increased from 47 to 187 L/ha.

The survival of two Rhizobium japonicum strains was determined in soils treated with 13 herbicides used in soybeans [Glycine max (L.) Merr.]. Survival in soils treated with concentrations equivalent to or 10-fold greater than those from field applications at recommended rates was compared to survival in untreated soil under controlled temperature and moisture conditions. Nine of the herbicides had transitory or no effects, regardless of rate. Acifluorfen {5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoic acid}, bentazon [3-(1-methylethyl)-(1H)-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide], fluchloralin [N-(2-chloroethyl)-2,6-dinitro-N-propyl-4-(trifluoromethyl)benzenamine], and dinoseb [2-(1-methylpropyl)-4,6-dinitrophenol] consistently reduced survival of one or both strains at rates 10-fold greater than recommended. At rates of application equivalent to suggested field use rates, effects on survival were reduced or eliminated. Reductions in survival attributable to herbicides were in some cases comparable to those obtained by a weekly wetting and drying of the soil, indicating that herbicide effects on R. japonicum may be equivalent in magnitude to effects due to environmental stress. The herbicides were not sufficiently toxic to reduce R. japonicum populations in soil to levels that would be likely to affect nodulation.