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In ovo cultivation of Microphallus primas (Trematoda: Microphallidae) metacercariae to ovigerous adults and the establishment of the life-cycle in the laboratory

Published online by Cambridge University Press:  06 April 2009

D. H. Saville  and
S. W. B. Irwin
D. H. Saville
Affiliation:
Department of Biological and Biomedical Sciences, University of Ulster at Jordanstown, Shore Road, Newtownabbey, Co. Antrim BT37 0QB, Northern Ireland
S. W. B. Irwin
Affiliation:
Department of Biological and Biomedical Sciences, University of Ulster at Jordanstown, Shore Road, Newtownabbey, Co. Antrim BT37 0QB, Northern Ireland
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Extract

This investigation reports for the first time the establishment of a trematode life-cycle in the laboratory using in ovo cultivation of the adult stage. Microphallus primas metacercarial cysts were removed from Carcinus maenas crabs and chemically excysted. Using a modification of the technique developed by Fried (1962) the released metacercariae were grown in fertile hen's eggs to ovigerous adult flukes. These were removed from the under surface of the chorio-allantoic membrane of chick embryos 5 days post-infection and the fluke eggs recovered were used to infect laboratory-reared Hydrobia ulvae snails. When challenged against parasite-free crabs the cercariae released from the experimentally infected snails gave rise to metacercarial cysts that were again identified as M. primas.

Keywords

Microphallus primasHydrobia ulvaeCarcinus maenasin ovo culturelife-cycle
Type
Research Article
Information
Parasitology , Volume 103 , Issue 3 , December 1991 , pp. 479 - 484
Copyright
Copyright © Cambridge University Press 1991

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References

Ahmad, R. A., James, B. L. & Kamis, A. B. (1986). Retention and egg production of Microphallus pygmaeus in mice: the influence of the adrenal cortex. Zeitschrift für Parasitenkunde 72, 479–85.CrossRefGoogle ScholarPubMed
Belopolskaya, M. M. (1957). Die Fauna der Trematodenlarven von Gammarus locusta L. aus der Ostsee. Trudy Leningradskogo. Obshchestua Estestvois pytatelei 73, 163–70.Google Scholar
Borysko, E. & Bang, F. B. (1953). The fine structure of the chorio-allantoic membrane of the normal chick embryo. Bulletin of the Johns Hopkins Hospital 92, 257–89.Google ScholarPubMed
Ching, H. L. (1963). The description and life-cycle of Maritrema laricola sp. (Trematoda: Microphallidae). Canadian Journal of Zoology 41, 881–8.CrossRefGoogle Scholar
Davies, C. & Smyth, J. D. (1979). Development of the metacercariae of Microphallus similis, in vitro and in the mouse. International Journal for Parasitology 9, 261–7.CrossRefGoogle Scholar
Dawes, B. (1968). The Trematoda. Cambridge: Cambridge University Press.Google Scholar
Deblock, S. (1971). Contribution à l'étude des Microphallidae Travassos, 1920. XXIV. Tentative de Phylogénie et de taxonomie. Bulletin du Muséum national d'histoire naturelle, 3e sér., No. 7, Zoologie 7, 353–468.Google Scholar
Fishbein, J. M., Fried, B. & Stableford, L. T. (1985). Histopathology of chick extra-embryonic membranes experimentally infected by Leucochloridiomorpha constantiae (Trematoda). Transactions of the American Microscopical Society 104, 267–71.CrossRefGoogle Scholar
Fried, B. (1962). Growth of Philophthalmus sp. (Trematoda) on the chorioallantois of the chick. Journal of Parasitology 48, 545–50.CrossRefGoogle Scholar
Fried, B. (1989). Cultivation of trematodes in chick embryos. Parasitology Today 5, 3–5.CrossRefGoogle ScholarPubMed
Fried, B. & Huffman, J. E. (1982). Excystation and development in the chick and on the chick chorioallantois of the metacercariae of Sphaeridiotrema globulus (Trematoda). International Journal for Parasitology 12, 427–31.CrossRefGoogle ScholarPubMed
Fried, B. & Nelson, P. D. (1978). Host–parasite relationships of Zygocotyle lunata (Trematoda) in the domestic chick. Parasitology 77, 49–55.CrossRefGoogle ScholarPubMed
Fried, B. & Pentz, L. (1983). Cultivation of excysted metacercariae of Echinostoma revolutum (Trematoda) in chick embryos. International Journal for Parasitology 13, 219–23.CrossRefGoogle ScholarPubMed
Irwin, S. W. B., Mckerr, G., Judge, B. A. & Moran, I. (1984). Studies on metacercarial excystment in Himasthla leptosoma (Trematoda: Echinostomatidae) and newly emerged metacercariae. International Journal for Parasitology 14, 415–21.CrossRefGoogle Scholar
Irwin, S. W. B. & Saville, D. H. (1988 a). Cultivation and development of Microphallus pygmaeus (Trematoda: Microphallidae) in fertile chick eggs. Parasitology Research 74, 396–8.CrossRefGoogle ScholarPubMed
Irwin, S. W. B. & Saville, D. H. (1988 b). An alternative method for the culture of Diplostomum spathaceum (Trematoda) in chick embryos. Journal of Parasitology 74, 504–5.CrossRefGoogle ScholarPubMed
James, B. L., Sannia, A. & Bowers, E. A. (1976). Parasites of birds and shellfish. In Problems of a Small Estuary (ed. Nelson-Smith, A. & Bridges, E. M.). Swansea: Institute of Marine Studies and Quadrant Press Ltd.Google Scholar