Synthetic biology is a broad and fast-moving field of innovation involving the design and construction of new biological parts, and the redesign of existing, natural biological systems to address real world problems. It has many potential applications that may change human relations to the natural world. Synthetic biology is virtually unknown to the conservation community. Based on a meeting in 2013 that brought together these two communities we consider first the differences between the two fields, and second the kinds of opportunities and risks that arise.

To identify forests of high priority for conservation in tropical dry forests of New Caledonia, Fiji, the Marquesas and Hawaii, we examined patterns of woody plant species richness (total, native and endemic) and threatened species (IUCN categorization and density) at the stand level, using Gentry's transect method. There were associations between total, native and endemic plant species richness in all four Pacific dry forest regions but we found no significant association with the presence or density of species listed on the IUCN Red List. Dry forests in New Caledonia and Hawaii merit the highest conservation priority in the Pacific, based on level of endemism and number of threatened species. The study sites that merit high conservation priority are Metzdorf, Nekoro and Pindai, in New Caledonia, Kokee and Kaupulehu, in Hawaii, and Vatia, in Fiji. New Caledonia and Fiji have a small dry forest extent and protected area extent compared with other dry forests in biodiversity hotspots. Although we identified priority areas for dry forest conservation, more comparative plot data, presence/absence data in fragments and regional geographical data are needed to adequately manage and protect dry forests in the Pacific.

A process for the review of significant trade in species listed in CITES Appendix II was established to address problems with the application of CITES Article IV. Article IV states that international trade in Appendix II species requires a Scientific Authority of the state of export to determine that such trade will not be detrimental to the species’ survival in the wild. Such a review process was initiated for the plant species Galanthus woronowii Losinsk. as a result of continuing uncertainty over the sustainability of international exports of the plant from wild populations in Georgia, and the Georgian Management Authority was tasked with determining and reporting whether export levels conformed to Article IV. Here we report on the project that was established to address that task. Nationwide field surveys conducted in 2009 led to the recommendation of a conservative annual export quota of 15 million plants for the sites surveyed. Cultivated populations held significant stocks of harvestable plants but with varied cultivation histories, especially in relation to the extent and nature of supplementary planting. Future work should continue to monitor harvesting from wild populations and identify solutions for local communities wishing to cultivate the plant for international trade.

Wenchengia alternifolia (Lamiaceae) is a plant species endemic to Hainan Island, China. Since the 1930s it was known from only four collections and was believed to be extinct until a remnant population was rediscovered in 2010. We conducted further field surveys during 2010–2012 but located only one population, with 66 individuals. W. alternifolia is restricted to the c. 1,500 m2 Shuangximu Valley, in a harsh microenvironment surrounded by plantations. As the population comprises < 50 mature individuals, we propose that W. alternifolia be categorized as Critically Endangered on the IUCN Red List, based on criterion D. We recommend that the remnant population and its habitat need urgent protection and monitoring and that ex situ conservation, for future reintroduction, should be implemented.

The distribution of the endemic threatened plant Camellia changii Ye (Family Theaceae) is restricted to a small area in southern China, and little else is known about its status in the wild. To provide information for the conservation of C. changii we investigated its distribution, population size and structure, and habitat, and assessed its conservation status. Surveys confirmed that the species grows in a narrow band along both sides of a 4 km long segment of a stream in Ehuangzhang Nature Reserve, under the discontinuous canopy of a secondary evergreen broadleaved forest on well-drained, acidic sandy loam soil. We found a total of 1,039 individuals of C. changii. The population has a high flowering rate but a low seed-setting rate. The population appears to be in decline because no seedlings and few young plants were evident. Our findings indicate that C. changii should be categorized as Critically Endangered on the IUCN Red List. We have recommended an integrated species-conservation plan for the species that includes patrolling the Ehuangzhang Nature Reserve to prevent plant removal, establishing an ex situ living collection that contains the entire wild genetic diversity (accomplished by grafting of short cuttings from all wild individuals), facilitating propagation for commercial use, and implementing reintroduction to augment the wild population.

The global population of jaguars Panthera onca has decreased significantly since the beginning of the 20th century. Given the scarcity of demographic and biological information, estimating population parameters is critical for the design of conservation measures. The jaguar's elusive behaviour makes it impossible to estimate and monitor populations by direct observation. We propose a non-invasive genetic sampling approach and demonstrate its potential for large-scale monitoring. Sex identification was optimized for faecal samples of jaguars and other felids. We also optimized a set of 11 microsatellite markers for reliable identification of individuals. We estimated the effectiveness of faecal sample genotyping in two distinct Brazilian biomes: the Pantanal and the semi-arid Caatinga. Almost 90% of the samples that were molecularly identified as jaguar (n = 90) were successfully genotyped and were assigned to 30 individuals. Genetic diversity was generally high but was significantly lower in the Caatinga population. We show that non-invasive genetic sampling can be a reliable tool to study population parameters and to monitor the genetic status of jaguar populations in different habitats. It may also be useful for future surveys of jaguars that address ecological, behavioural and conservation issues, and could provide a baseline for non-invasive genetic studies of other wild felid populations.

Tigers Panthera tigris are highly threatened and continue to decline across their entire range. Actions to restore and conserve populations need to be based on science but, in South-east Asia, information on ecology and behaviour of tigers is lacking. This study reports the relationship between the home range size of female tigers and prey abundance, using data from radio-collared tigers in Huai Kha Khaeng Wildlife Sanctuary, Thailand, and published data from other studies. A total of 11 tigers, four males and seven females, were fitted with global positioning system collars, to estimate home ranges using 95 and 100% minimum convex polygons (MCP). Prey abundance was estimated by faecal accumulation rates. The mean home range size of male tigers was 267 and 294 km2 based on 95 and 100% MCPs, respectively; the mean female home range size was 70 and 84 km2, respectively. Territories of male and female tigers had little overlap, which indicated both sexes were territorial. Mean densities of the prey species sambar Rusa unicolor, barking deer Muntiacus muntjac and large bovids were 7.5, 3.5 and 3.0 km−2, respectively. When female home range size and prey abundance were compared at six locations in Thailand, and at other sites in India, Nepal, Bangladesh and Russia, a significant negative correlation was found between prey abundance and home range size. Monitoring this relationship can provide managers with metrics for setting conservation goals.

During 2007–2009 we conducted a survey of attacks by wild carnivores on the livestock of the Maasai and Sonjo tribes in the eastern Serengeti ecosystem of northern Tanzania. Local enumerators systematically recorded incidents of carnivore predation on livestock and their data were quantified by us, with the aid of District Game Officers or trusted local people. The annual rate of attack by African wild dogs Lycaon pictus was significantly higher (1.42 animals per 1,000 livestock) in the Sonjo tribal area than in the Maasai tribal area (0.72 animals per 1,000 livestock). In the Maasai tribal area there was a significant amount of predation by leopards Panthera pardus and spotted hyaenas Crocuta crocuta. In both tribal areas sheep Ovis aries and goats Capra aegagrus hircus were subject to predation more frequently than cattle. Attacks on livestock by wild dogs occurred most frequently in the afternoon and evening, whereas other carnivores generally attacked livestock at night. Sheep and goats were most frequently attacked by most carnivores during the long rainy season. Cattle Bos primigenius were most frequently attacked by wild dogs and leopards during the long dry season and by lions Panthera leo during the long rainy season, whereas spotted hyaenas killed cattle most frequently during the short rainy season.

The African lion Panthera leo is an iconic species but it has faced dramatic range reductions and possibly as few as 30,000 individuals remain in the wild. In the absence of detailed ground-based surveys, lion populations may be estimated using regression models based on prey biomass availability but these often overestimate lion densities as a result of a variety of compounding factors. Anthropogenic factors can be key drivers of lion population dynamics and in areas with high human impact lion numbers may be significantly lower than those predicted by prey biomass models. This was investigated in two protected areas in Zimbabwe, where lion population densities were found to be significantly lower than would have been predicted by prey-availability models. High hunting quotas either within or around the protected areas are the most likely cause of the low lion numbers, with quotas in some areas being as high as seven lions per 1,000 km2 in some years. Other factors, including persecution, poisoning and problem animal control, as well as disease and competition with spotted hyaenas Crocuta crocuta, are also discussed.

Despite > 60 years of conservation in Uganda's national parks the populations of lions and spotted hyaenas in these areas have never been estimated using a census method. Estimates for some sites have been extrapolated to other protected areas and educated guesses have been made but there has been nothing more definitive. We used a lure count analysis method of call-up counts to estimate populations of the lion Panthera leo and spotted hyaena Crocuta crocuta in the parks where reasonable numbers of these species exist: Queen Elizabeth Protected Area, Murchison Falls Conservation Area and Kidepo Valley National Park. We estimated a total of 408 lions and 324 hyaenas for these three conservation areas. It is unlikely that other conservation areas in Uganda host > 10 lions or > 40 hyaenas. The Queen Elizabeth Protected Area had the largest populations of lions and hyaenas: 140 and 211, respectively. It is estimated that lion numbers have declined by 30% in this protected area since the late 1990s and there are increasing concerns for the long-term viability of both species in Uganda.