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Bio-inspired synthesis of superparamagnetic iron oxide nanoparticles for enhanced in vitro anticancer therapy

Published online by Cambridge University Press:  19 March 2018

Thangavel Shanmugasundaram ,
Manikkam Radhakrishnan ,
Arasu Poongodi ,
Krishna Kadirvelu  and
Ramasamy Balagurunathan
Thangavel Shanmugasundaram
Affiliation:
Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India DRDO-BU Centre for Life Sciences, Bharathiar University Campus, Coimbatore 641 046, Tamil Nadu, India
Manikkam Radhakrishnan
Affiliation:
Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology (Deemed to be University), Jeppiaar Nagar, Chennai 600 119, Tamil Nadu, India
Arasu Poongodi
Affiliation:
Department of Biochemistry, Sri Ramachandra University, Chennai 600 116, Tamil Nadu, India
Krishna Kadirvelu
Affiliation:
DRDO-BU Centre for Life Sciences, Bharathiar University Campus, Coimbatore 641 046, Tamil Nadu, India
Ramasamy Balagurunathan*
Affiliation:
Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India
*
Address all correspondence to Ramasamy Balagurunathan at [email protected]
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Abstract

Superparamagnetic iron oxide nanoparticles (SPIONPs) are successfully synthesized in this study by co-precipitation method using actinobacterial metabolites as reducing agent. Physicochemical and morphological features of the nanoparticles (NPs) are analyzed by Fourier-transform infrared spectroscopy, x-ray-based techniques, vibrating sample magnetometer, thermal gravimetric analysis, and electron microscopic analysis, with an average size of 15–30 nm. Anticancer activity of the magnetite-NPs is systematically evaluated on HeLa cells using MTT assay, Hoechst nuclear staining, acridine orange/ethidium bromide dual staining and flow cytometric analysis. The obtained results open a new route for biosynthesis of SPIONPs, which to be used for various biomedical applications, particularly in cancer therapy.

Type
Research Letters
Information
MRS Communications , Volume 8 , Issue 2 , June 2018 , pp. 604 - 609
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Copyright
Copyright © Materials Research Society 2018 

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