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Ultrastructural Analyses of Living Animal Organs Prepared by “in vivo Cryotechnique” for Electron Microscopy

Published online by Cambridge University Press:  02 July 2020

S. Ohno ,
I. Takayama ,
N. Terada ,
Y. Fujii ,
H. Ueda  and
T. Baba
S. Ohno
Affiliation:
Department of Anatomy, Yamanashi Medical University, 1110 Shimokato, Tamaho, Yamanashi, 409-3898, Japan
I. Takayama
Affiliation:
Department of Anatomy, Yamanashi Medical University, 1110 Shimokato, Tamaho, Yamanashi, 409-3898, Japan
N. Terada
Affiliation:
Department of Anatomy, Yamanashi Medical University, 1110 Shimokato, Tamaho, Yamanashi, 409-3898, Japan
Y. Fujii
Affiliation:
Department of Anatomy, Yamanashi Medical University, 1110 Shimokato, Tamaho, Yamanashi, 409-3898, Japan
H. Ueda
Affiliation:
Department of Anatomy, Yamanashi Medical University, 1110 Shimokato, Tamaho, Yamanashi, 409-3898, Japan
T. Baba
Affiliation:
Department of Anatomy, Yamanashi Medical University, 1110 Shimokato, Tamaho, Yamanashi, 409-3898, Japan
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Abstract

It is well known that hemodynamic factors, such as blood pressure and flow, exert an important influence on native morphology of various animal organs. However, information about the significance of ultrastructures which are revealed by conventional preparation methods has been limited, because routine chemical fixation of cells and tissues takes considerable time, during which their morphology could easily change. Therefore, morphological studies with routine immersion or perfusion fixation have not revealed real ultrastructures of functioning animal organs with normal blood circulation.2 The ultimate goal of morphological study is that all features to be examined should reflect the physiological meaning under investigation. For that purpose, the preservation of cells and tissues in functioning organs is necessary for studies to define their ultrastructures. Most cryotechniques have been based on the use of prior excised tissues. Such small specimens are commonly frozen within several seconds following the excision of the tissues.

Type
Cryoimmobilization, Freeze Substitution and Cryoem (Organized by S. Erlandsen)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 730 - 731
Copyright
Copyright © Microscopy Society of America 2001

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