Abstract: Using standard square mesh grids as supports, we have prepared sol-gel membranes from a functionalized silane precursor, bis[3-(triethoxysilyl)propyl]tetrasulfide in various organic solvents. These mesh-supported membranes allow direct characterization of their microstructure and morphology by transmission electron microscopy (TEM). Homogeneous membranes without prominent microstructural features (or featureless) at nanometer scale were obtained from dimethyl formamide (DMF), isopropanol, and butanol solutions. However, highly porous membranes were produced from DMF and ethylene glycol binary system. Heterogeneous membranes filled with nanoparticles were formed from dimethyl sulfoxide solution. The featureless sol-gel membranes with tunable performances provide a useful alternative as supporting films for TEM applications.