Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-27T00:44:02.787Z Has data issue: false hasContentIssue false

Transforming FIB-SEM for Large Volume Imaging: A Powerful Discovery Platform for Biological Sciences

Published online by Cambridge University Press:  30 July 2020

C. Shan Xu*
Affiliation:
Janelia Research Campus, HHMI, Ashburn, Virginia, United States

Abstract

Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
3D Scanning Electron Microscopy Imaging of Biological Samples
Copyright
Copyright © Microscopy Society of America 2020

References

Xu, C. S., Hayworth, K. J., Lu, Z., et al. , “Enhanced FIB-SEM systems for large-volume 3D imaging,eLife, 6: e25916 (2017).10.7554/eLife.25916CrossRefGoogle Scholar
Xu, C. S., Januszewski, M., Lu, Z., et al. , “A connectome of the adult Drosophila central brain,bioRxiv, 2020.01.21.911859 (2020).Google Scholar
Wu, Y., Whiteus, C., Xu, C. S., et al. , “Contacts between the endoplasmic reticulum and other membranes in neurons,PNAS 114, E4859 (2017).10.1073/pnas.1701078114CrossRefGoogle Scholar
Xu, C. S., Pang, S., Hayworth, K. J., et al. , “Enabling FIB-SEM Systems for Large Volume Connectomics and Cell Biology,bioRxiv 852863, (2019).Google Scholar
Hoffman, D. P., Shtengel, G., Xu, C. S., et al. , “Correlative three-dimensional super-resolution and block face electron microscopy of whole vitreously frozen cells,Science 367, eaaz5357 (2020).10.1126/science.aaz5357CrossRefGoogle Scholar
Takemura, S., Xu, C.S., Lu, Z., et al. , “Synaptic circuits and their variations within different columns in the visual system of Drosophila,PNAS 112, 13711 (2015).10.1073/pnas.1509820112CrossRefGoogle Scholar
Gao, R., Asano, S. M., Upadhyayula, S., et al. , “Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution,Science 363, eaau8302 (2019).10.1126/science.aau8302CrossRefGoogle Scholar
Nixon-Abell, J., Obara, C. J., Weigel, A. V., et al. , “Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER,Science 354, 433 (2016).10.1126/science.aaf3928CrossRefGoogle Scholar
Ioannou, M. S., Jackson, J., Sheu, S., et al. , “Neuron-Astrocyte Metabolic Coupling Protects against Activity-Induced Fatty Acid Toxicity,Cell 177, 1522 (2019).10.1016/j.cell.2019.04.001CrossRefGoogle Scholar
Chang, C., Weigel, A. V., Ioannou, M. S., et al. , “Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III,J. Cell Biol. 218 (8), 2583 (2019).10.1083/jcb.201902061CrossRefGoogle Scholar