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Surface Charge and Carbon Contamination on an Electron-Beam-Irradiated Hydroxyapatite Thin Film Investigated by Photoluminescence and Phase Imaging in Atomic Force Microscopy

Published online by Cambridge University Press:  09 April 2014

Radu Hristu ,
Denis E. Tranca ,
Stefan G. Stanciu ,
Maros Gregor ,
Tomas Plecenik ,
Martin Truchly ,
Tomas Roch ,
Syed A. M. Tofail  and
George A. Stanciu Open the ORCID record for George A. Stanciu [Opens in a new window]
Radu Hristu
Affiliation:
Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania
Denis E. Tranca
Affiliation:
Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania
Stefan G. Stanciu
Affiliation:
Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania
Maros Gregor
Affiliation:
Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University, 842 48 Bratislava, Slovakia
Tomas Plecenik
Affiliation:
Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University, 842 48 Bratislava, Slovakia
Martin Truchly
Affiliation:
Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University, 842 48 Bratislava, Slovakia
Tomas Roch
Affiliation:
Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University, 842 48 Bratislava, Slovakia
Syed A. M. Tofail
Affiliation:
Materials and Surface Science Institute, University of Limerick, Limerick, Ireland
George A. Stanciu*
Affiliation:
Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania
*
*Corresponding author: [email protected]
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Abstract

The surface properties of hydroxyapatite, including electric charge, can influence the biological response, tissue compatibility, and adhesion of biological cells and biomolecules. Results reported here help in understanding this influence by creating charged domains on hydroxyapatite thin films deposited on silicon using electron beam irradiation and investigating their shape, properties, and carbon contamination for different doses of incident injected charge by two methods. Photoluminescence laser scanning microscopy was used to image electrostatic charge trapped at pre-existing and irradiation-induced defects within these domains, while phase imaging in atomic force microscopy was used to image the carbon contamination. Scanning Auger electron spectroscopy and Kelvin probe force microscopy were used as a reference for the atomic force microscopy phase contrast and photoluminescence laser scanning microscopy measurements. Our experiment shows that by combining the two imaging techniques the effects of trapped charge and carbon contamination can be separated. Such separation yields new possibilities for advancing the current understanding of how surface charge influences mediation of cellular and protein interactions in biomaterials.

Keywords

hydroxyapatitesurface chargecarbon contaminationlaser scanning microscopyphotoluminescenceatomic force microscopy
Type
Materials Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 2 , April 2014 , pp. 586 - 595
Copyright
© Microscopy Society of America 2014 

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