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The Structure of Ppv0l, A Marine Algal Virus with a T=219 Capsid

Published online by Cambridge University Press:  02 July 2020

X. Yan ,
P. Chipman ,
T. Castberg ,
G. Bratbak  and
T. S. Baker
X. Yan
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907
P. Chipman
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907
T. Castberg
Affiliation:
Department of Microbiology, University of Bergen, N-5020, Bergen, Norway
G. Bratbak
Affiliation:
Department of Microbiology, University of Bergen, N-5020, Bergen, Norway
T. S. Baker
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907
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Abstract

Phaeocystispouchetii virus (PpV0l), first isolated from Norwegian coastal water in 1995, infects and lyses the haptophyte Phaeocystis pouchetii Lagerheim. PpV0l has not yet been assigned to a family. Phaeocystis may play an important role in ocean climate control and PpV0l infection could alter the amount of this haptophyte. The virion contains a dsDNA genome (∼450 kbps) encapsulated within a large icosahedral shell. The most abundant protein has a mass of 59 kDa. More than five minor polypeptides with masses ranging from 30 to 50 kDa have also been identified. We have used cryo-electron microscopy (cryo-EM) and three-dimensional (3D) image reconstruction methods to examine the native morphology of PpV0l.

Purified PpV0l samples were prepared for Cryo-EM as described. Images were recorded on film at 33,000x nominal magnification in a Philips CM300 FEG microscope at 300kV, at 1.0μm to 2.0μm under focus, and with an electron dose of 2200e-/nm2 (Fig. 1A).

Type
Microbiology
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 748 - 749
Copyright
Copyright © Microscopy Society of America 2001

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8.We thank R. Ashmore for assistance with programming. Work supported in part by grants from the NIH (GM-33050) and AI-45976 and NSF (MCB-9527131) to T.S.B. and by a grant from The Research Council of Norway (project no.121425/420) and an award to Purdue Structural Biology Group from the Keck Foundation.Google Scholar