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Streptavidin Monolayer-Crystal Affinity Grids: A Step Toward Controlling What Happens During Cryo-EM Sample Preparation

Published online by Cambridge University Press:  04 August 2017

Robert M. Glaeser ,
Bong-Gyoon Han  and
Jamie H. D. Cate
Robert M. Glaeser
Affiliation:
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA, USA
Bong-Gyoon Han
Affiliation:
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA, USA
Jamie H. D. Cate
Affiliation:
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA, USA Department of Molecular and Cell Biology, California Institute of Quantitative Biosciences, and Department of Chemistry, University of California, Berkeley, CA, USA

Abstract

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Abstract
Information
Microscopy and Microanalysis , Volume 23 , Supplement S1: Proceedings of Microscopy & Microanalysis 2017 , July 2017 , pp. 820 - 821
Copyright
© Microscopy Society of America 2017 

References

[1] Glaeser, RM & Han, BG Biophysics Reports (2016) p.1.Google Scholar
[2] Yoshimura, H, Scheybani, T & Baumeister, W Langmuir 10 1994). p. 3290.CrossRefGoogle Scholar
[3] Han, BG, et al, J Struct Biol 195 2016). p. 238.CrossRefGoogle Scholar
[4] The authors acknowledge funding from NIH grants P01 GM051487 and R01 GM065050. We thank Dr. Arto Pulk for providing the sample of randomly biotinylated 70S ribosome particles.Google Scholar