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Similarities and Differences in Cellular Processing of Biologically Relevant Nanoparticles

Published online by Cambridge University Press:  30 July 2020

Burcu Kepsutlu  and
James McNally
Burcu Kepsutlu
Affiliation:
Varex Imaging, Walluf, Hessen, Germany
James McNally
Affiliation:
Helmholtz Zentrum Berlin, Berlin, Berlin, Germany

Abstract

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Nanoparticles are endocytosed and trafficked within cells by selecting one or more of the cell's intrinsic processing pathways. However, general rules which drive this selection have not yet been defined. Therefore, each nanoparticle has to be investigated separately to determine its endocytosis and intracellular trafficking pathways. By using cryo SXT to investigate the cellular interaction of two different nanoparticles, we have uncovered some general rules about nanoparticle interactions with cells. Our results highlight not only the importance of cryo-SXT for nanoparticle investigation in the medical field, but also demonstrate that a well-controlled analysis makes it possible to discern general rules about cell-nanoparticle interactions.

Type
Biological Soft X-Ray Tomography
Information
Microscopy and Microanalysis , Volume 26 , Supplement S2 , August 2020 , pp. 3010 - 3012
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Copyright
Copyright © Microscopy Society of America 2020

References

Kepsutlu, B. (2019) Interaction of biologically relevant nanoparticles with cells studied by cryo soft X-ray tomography. PhD dissertation. Humboldt University Berlin.Google Scholar
Kepsutlu, B. et al. (2020) Cells undergo major changes in the quantity of cytoplasmic organelles after uptake of gold nanoparticles with biologically relevant surface coatings. ACS Nano 14:2248-2264.10.1021/acsnano.9b09264CrossRefGoogle ScholarPubMed
Aon, M.A. et al. (2014) Mitochondrial and cellular mechanisms for managing lipid excess. Front. Physiol. 5: 282.10.3389/fphys.2014.00282CrossRefGoogle ScholarPubMed
Bus, T. et al. (2018) The great escape: how cationic polyplexes overcome the endosomal barrier. J. Mater. Chem. B 6:6904-6918.10.1039/C8TB00967HCrossRefGoogle ScholarPubMed