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Optimizing Point Spread Functions to Discern Highly Overlapping Emission Spectra

Published online by Cambridge University Press:  30 July 2021

Sanduni Fernando ,
Jason Martineau ,
Erik Jorgensen  and
Jordan Gerton
Sanduni Fernando
Affiliation:
Department of Physics and Astronomy, University of Utah, Salt Lake City, USA, SALT LAKE CITY, Utah, United States
Jason Martineau
Affiliation:
Department of Physics and Astronomy, University of Utah, Salt Lake City, USA, United States
Erik Jorgensen
Affiliation:
Department of Biology, University of Utah, Salt Lake City, USA, United States
Jordan Gerton
Affiliation:
Department of Physics and Astronomy, University of Utah, Salt Lake City, USA, United States

Abstract

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Type
Frontiers in Fluorescence Lifetime and Super-resolution Imaging of Biological Structures and Dynamics
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 864 - 867
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Shechtman, Y., Weiss, L. E., Backer, A. S., Lee, M. Y. and Moerner, W. E., "Multicolour localization microscopy by point-spread-function engineering," Nature Photonics, vol. 10, no. 2016, p. 590–594, 2016.Google Scholar
Shechtman, Y., Weiss, L. E., Backer, A. S., Sahl, S. J. and Moerner, W. E., "Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions," Nano Letters, vol. 15, no. 6, p. 4194–4199, 2015.CrossRefGoogle Scholar
Broeken, J., Rieger, B. and Stallinga, S., "Simultaneous measurement of position and color of single fluorescent emitters using diffractive optics," Optics Letters, vol. 11, no. 3352-3355, p. 39, 2014.Google Scholar
Zhou, Y., Zickus, V., Harvey, A. R. and Zammit, P., "Double-Cubic Point Spread Function for 3D Extended-Depth Localization Microscopy," in Imaging and Applied Optics, 2018.Google Scholar
Shechtman, Y., Sahl, S. J., Backer, A. S. and Moerner, W., "Optimal Point Spread Function Design for 3D Imaging," Physical Review Letters, vol. 113, no. 13, p. 133902, 2014.CrossRefGoogle Scholar
Martineau, J. T., Menon, R., Jorgensen, E. M. and Gerton, J., "A Proposed Method for Optimizing the Spectral Discernibility of Engineered Point-spread Functions for Localization Microscopy," in Microscopy and Microanalysis , Volume 25 , Supplement (S2) , pp. 1232 - 1233, 2019.CrossRefGoogle Scholar
Brownlee, J., "How to Calculate the KL Divergence for Machine Learning," 18 October 2019. [Online]. Available: https://machinelearningmastery.com. [Accessed 20 December 2020].Google Scholar
Nielson, F., "Chernoff information of exponential families," arXiv 1102.2684v1 , 2011.Google Scholar
Alzaid, A. A. and Omair, M. A., "On The Poisson Difference Distribution," The Bulletin of the Malaysian Mathematical Society, 2009.Google Scholar
Kirshner, H., Aguet, F., Sage, D. and Unser, M., "3-D PSF fitting for fluorescence microscopy: implementation and," Journal of Microscopy, vol. 249, no. Pt 1 2013, p. 13–25, 2012.CrossRefGoogle Scholar