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The Influence of Detector Quality and Throughput for Cryo-EM of Challenging Proteins

Published online by Cambridge University Press:  30 July 2020

Joshua Mendez  and
Scott Stagg
Joshua Mendez
Affiliation:
Florida State University, Tallahassee, Florida, United States
Scott Stagg
Affiliation:
Florida State University, Tallahassee, Florida, United States

Abstract

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Type
Image Processing Developments in Cryo-EM
Information
Microscopy and Microanalysis , Volume 26 , Supplement S2 , August 2020 , pp. 2318 - 2320
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Copyright
Copyright © Microscopy Society of America 2020

References

McMullan, G., Chen, S., Henderson, R., and Faruqi, A. R., “Detective quantum efficiency of electron area detectors in electron microscopy,” Ultramicroscopy, vol. 109, no. 9, pp. 1126–1143, Aug. 2009, doi: 10.1016/j.ultramic.2009.04.002.CrossRefGoogle ScholarPubMed
Herzik, M. A., Wu, M., and Lander, G. C., “Achieving better than 3 Å resolution by single particle cryo-EM at 200 keV,” Nat Methods, vol. 14, no. 11, pp. 1075–1078, Nov. 2017, doi: 10.1038/nmeth.4461.CrossRefGoogle ScholarPubMed
Henderson, R., “The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules,” Quarterly Reviews of Biophysics, vol. 28, no. 2, pp. 171–193, May 1995, doi: 10.1017/S003358350000305X.CrossRefGoogle ScholarPubMed
Khoshouei, M., Radjainia, M., Baumeister, W., and Danev, R., “Cryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plate,” Nat Commun, vol. 8, no. 1, pp. 1–6, Jun. 2017, doi: 10.1038/ncomms16099.CrossRefGoogle ScholarPubMed
Herzik, M. A., Wu, M., and Lander, G. C., “High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM,” Nat Commun, vol. 10, no. 1, pp. 1–9, Mar. 2019, doi: 10.1038/s41467-019-08991-8.CrossRefGoogle ScholarPubMed
Mendez, J. H., Mehrani, A., Randolph, P., and Stagg, S., “Throughput and resolution with a next-generation direct electron detector,” IUCrJ, vol. 6, no. 6, pp. 1007–1013, Nov. 2019, doi: 10.1107/S2052252519012661.CrossRefGoogle ScholarPubMed
Henderson, R. and McMullan, G., “Problems in obtaining perfect images by single-particle electron cryomicroscopy of biological structures in amorphous ice,” Microscopy (Oxf), vol. 62, no. 1, pp. 43–50, Feb. 2013, doi: 10.1093/jmicro/dfs094.CrossRefGoogle ScholarPubMed
Massover, W. H., “Ultrastructure of ferritin and apoferritin: A review,” Micron, vol. 24, no. 4, pp. 389–437, Jan. 1993, doi: 10.1016/0968-4328(93)90005-L.CrossRefGoogle Scholar
Russo, C. J. and Passmore, L. A., “Ultrastable gold substrates for electron cryomicroscopy,” Science, vol. 346, no. 6215, pp. 1377–1380, Dec. 2014, doi: 10.1126/science.1259530.CrossRefGoogle ScholarPubMed