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In-Chamber Reel-to-Reel System for Random-Access Volume Electron Microscopy

Published online by Cambridge University Press:  04 August 2017

S. Mikula
S. Mikula*
Affiliation:
Electrons - Photons - Neurons, Max-Planck Institute of Neurobiology, Martinsried, Germany.

Abstract

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Type
Abstract
Information
Microscopy and Microanalysis , Volume 23 , Supplement S1: Proceedings of Microscopy & Microanalysis 2017 , July 2017 , pp. 154 - 155
Copyright
© Microscopy Society of America 2017 

References

[1] Mikula, S. Progress Towards Mammalian Whole-Brain Cellular Connectomics. Frontiers in Neuroanatomy 62 2016.Google Scholar
[2] Schalek, R., et al Development of High-Throughput, High-Resolution 3D Reconstruction of Large-Volume Biological Tissue Using Automated Tape Collection Ultramicrotomy and Scanning Electron Microscopy. Microscopy and Microanalysis 17(Supplement S2), 966–967, 2011.Google Scholar
[3] Hayworth, K. & Hayworth, A. Methods and Apparatuses for the Automated Production, Collection, Handling, and Imaging of Large Numbers of Serial Tissue Sections. http://www.google.com/patents/US20060008790. (2006).Google Scholar
[4] Own, C.S., et al Reel-to-Reel Electron Microscopy: Latency-Free Continuous Imaging of Large Sample Volumes. Microscopy and Microanalysis 21(S3), 157–158, 2015.Google Scholar
[5] Mikula, S. & Denk, W. High-Resolution Whole-Brain Staining for Electron Microscopic Circuit Reconstruction. Nature Methods 12(6), 541–546, 2015.CrossRefGoogle ScholarPubMed
[6] The author acknowledges funding from the Max Planck Society and thanks Winfried Denk for his support, Pat Brey, Ken Hayworth and Yoshiyuki Kubota for many useful discussions, Juergen Tritthardt for electronics support and Debbie Zapoglou for CAD support.Google Scholar