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The Effects of Spaceflight Conditions on Calcium-Dependent Secretion and Cytoskeletal Organization During Fertilization and Cell Divisions In the Sea Urchin Lytechinus Pictus

Published online by Cambridge University Press:  02 July 2020

Heide Schatten ,
Amitabha Chakrabarti ,
Howard Levine ,
Mario Runco ,
Ken Anderson  and
Meghan Taylor
Heide Schatten
Affiliation:
Department of Veterinary Pathobiology, University of Missouri-Columbia
Amitabha Chakrabarti
Affiliation:
Department of Veterinary Pathobiology, University of Missouri-Columbia
Howard Levine
Affiliation:
Gravitational Biology Laboratory, Dynamac Corp., Kennedy Space Center, FL32899
Mario Runco
Affiliation:
Astronaut Office, Johnson Space Center, NASA, Houston, TX77058
Ken Anderson
Affiliation:
Flight Development Group, Bionetics Corp., Kennedy Space Center, FL32899
Meghan Taylor
Affiliation:
Department of Veterinary Pathobiology, University of Missouri-Columbia
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Extract

Calcium loss and muscle atrophy are two of the main metabolic changes experienced by astronauts and crew members during exposure to microgravity in space. To investigate the effects of spaceflight on calcium-dependent secretion and cytoskeletal formation in a less complex system we utilized sea urchin eggs and embryos which were fertilized and cultured under spaceflight conditions during the STS-77 shuttle mission. Sea urchin eggs were fertilized and cultured in the newly developed aquatic research facility (ARF) which allowed culture of eggs and embryos in microgravity and in a 1g centrifuge in space. This allowed analysis of the comparison of microgravity and 1g spaceflight treatments with samples cultured on ground. Eggs and embryos were maintained in Standard Container Assemblies (SCAs) with identical sets prepared for culture in microgravity, and at 1g in the middeck compartment of the shuttle Endeavor, as well as for ground observations at the Kennedy Space Center.

Type
Applications of Imaging Techniques to the Study of Embryological Development
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1070 - 1071
Copyright
Copyright © Microscopy Society of America

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References

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