Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-27T02:43:48.814Z Has data issue: false hasContentIssue false

CryoEM Structure of Drosophila Flight Muscle Thick Filaments at 7Å Resolution

Published online by Cambridge University Press:  30 July 2020

Nadia Daneshparvar
Affiliation:
Florida State University, Tallahassee, Florida, United States
Hamidreza Rahmani
Affiliation:
Florida State University, Tallahassee, Florida, United States
Dianne Taylor
Affiliation:
Florida State University, Tallahassee, Florida, United States
Thomas O'Leary
Affiliation:
University of Vermont College of Medicine, Burlington, Vermont, United States
Fatemeh Abbasi Yeganeh
Affiliation:
Florida State University, Tallahassee, Florida, United States
Micheal Previs
Affiliation:
University of Vermont College of Medicine, Burlington, Vermont, United States
Kenneth Taylor
Affiliation:
Florida State University, Tallahassee, Florida, United States

Abstract

Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
3D Structures: From Macromolecular Assemblies to Whole Cells (3DEM FIG)
Copyright
Copyright © Microscopy Society of America 2020

References

Farman, G.P., Miller, M.S., Reedy, M.C., Soto-Adames, F.N., Vigoreaux, J.O., Maughan, D.W., and Irving, T.C. (2009). Phosphorylation and the N-terminal extension of the regulatory light chain help orient and align the myosin heads in the Drosophila flight muscle. J Struct Biol 168, 240-249.10.1016/j.jsb.2009.07.020CrossRefGoogle ScholarPubMed
Levine, R.J., Kensler, R.W., Yang, Z., and Sweeney, H.L. (1995). Myosin regulatory light chain phosphorylation and the production of functionally significant changes in myosin head arrangement on striated muscle thick filaments. Biophys J 68, 224S.Google ScholarPubMed
Scheres, S.H. (2012). A Bayesian view on cryo-EM structure determination. Journal of molecular biology 415, 406-418.10.1016/j.jmb.2011.11.010CrossRefGoogle ScholarPubMed
Wendt, T., Taylor, D., Trybus, K.M., and Taylor, K. (2001). Three-dimensional image reconstruction of dephosphorylated smooth muscle heavy meromyosin reveals asymmetry in the interaction between myosin heads and placement of subfragment 2. Proc Natl Acad Sci U S A 98, 4361-4366.10.1073/pnas.071051098CrossRefGoogle ScholarPubMed
Ayer, G., and Vigoreaux, J.O. (2003). Flightin is a myosin rod binding protein. Cell Biochem Biophys 38, 41-54.10.1385/CBB:38:1:41CrossRefGoogle ScholarPubMed
Ayme-Southgate, A., and Southgate, R. (2006). Projectin, the elastic protein of the C-filaments. In Nature's versatile engine insect flight muscle inside and out, Vigoreaux, J., ed. (Georgetown, TX: Landes Bioscience/Eurekah.com), pp. 167-176.10.1007/0-387-31213-7_13CrossRefGoogle Scholar
Grant, T., Rohou, A., and Grigorieff, N. (2018). cisTEM, user-friendly software for single-particle image processing. Elife 7.10.7554/eLife.35383CrossRefGoogle ScholarPubMed
Hu, Z., Taylor, D.W., Reedy, M.K., Edwards, R.J., and Taylor, K.A. (2016). Structure of myosin filaments from relaxed Lethocerus flight muscle by cryo-EM at 6 Å resolution. Sci Adv 2, e1600058.10.1126/sciadv.1600058CrossRefGoogle ScholarPubMed
Irving, M. (2017). Regulation of Contraction by the Thick Filaments in Skeletal Muscle. Biophys J 113, 2579-2594.10.1016/j.bpj.2017.09.037CrossRefGoogle ScholarPubMed
Qiu, F., Brendel, S., Cunha, P.M., Astola, N., Song, B., Furlong, E.E., Leonard, K.R., and Bullard, B. (2005). Myofilin, a protein in the thick filaments of insect muscle. J Cell Sci 118, 1527-1536.10.1242/jcs.02281CrossRefGoogle ScholarPubMed