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Cryo-EM and Molecular Biology Approaches for Characterizing Flagellar Filament Structures of Caulobacter crescentus

Published online by Cambridge University Press:  30 July 2020

Juan Sanchez ,
Nicoleta Ploscariu ,
Eric Montemayor ,
Daniel Parrell ,
Gan Li ,
Rebecca Dillard ,
Conrad Shebelut  and
Elizabeth Wright
Juan Sanchez
Affiliation:
University of Wisconsin - Madison, Madison, Wisconsin, United States
Nicoleta Ploscariu
Affiliation:
University of Wisconsin - Madison, Madison, Wisconsin, United States
Eric Montemayor
Affiliation:
University of Wisconsin - Madison, Madison, Wisconsin, United States
Daniel Parrell
Affiliation:
University of Wisconsin - Madison, Madison, Wisconsin, United States
Gan Li
Affiliation:
University of Wisconsin - Madison, Madison, Wisconsin, United States
Rebecca Dillard
Affiliation:
Emory University, Atlanta, Georgia, United States
Conrad Shebelut
Affiliation:
Emory University, Atlanta, Georgia, United States
Elizabeth Wright
Affiliation:
University of Wisconsin - Madison, Madison, Wisconsin, United States

Abstract

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Type
3D Structures: From Macromolecular Assemblies to Whole Cells (3DEM FIG)
Information
Microscopy and Microanalysis , Volume 26 , Supplement S2 , August 2020 , pp. 806 - 808
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Copyright
Copyright © Microscopy Society of America 2020

References

Faulds-Pain, A., Birchall, C., Aldridge, C., et al. ., Flagellin redundancy in Caulobacter crescentus and its implications for flagellar filament assembly, J Bacteriol 193(11) (2011) 2695-707.10.1128/JB.01172-10CrossRefGoogle ScholarPubMed
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Casanal, A., Lohkamp, B., Emsley, P., Current Developments in Coot for Macromolecular Model Building of Electron Cryo-microscopy and Crystallographic Data, Protein Sci (2019).Google Scholar
Liebschner, D., Afonine, P.V., Baker, M.L., et al. ., Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix, Acta Crystallogr D Struct Biol 75(Pt 10) (2019) 861-877.10.1107/S2059798319011471CrossRefGoogle ScholarPubMed
This research was supported in part by funds from the University of Wisconsin, Madison, Emory University, Children's Healthcare of Atlanta, the Emory Center for AIDS Research, the Georgia Research Alliance, Human Frontiers Science Program, National Institutes of Health (R01GM104540 and R01GM104540-03S1), and the National Science Foundation (0923395) to E.R.W. Cryo-EM data collection using the SECM resource at Florida State University was supported by National Institutes of Health grant U24GM123456. The authors gratefully acknowledge use of facilities and instrumentation at the Emory University Robert P. Apkarian Integrated Electron Microscopy Core and at the UW-Madison Wisconsin Centers for Nanoscale Technology (wcnt.wisc.edu) partially supported by the NSF through the University of Wisconsin Materials Research Science and Engineering Center (DMR-1720415).Google Scholar