Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-26T08:54:46.306Z Has data issue: false hasContentIssue false
  • English
  • Français

Cryo-electron Tomography Workflows for Quantitative Analysis of Actin Networks Involved in Cell Migration

Published online by Cambridge University Press:  30 July 2020

Florian Fäßler ,
Georgi Dimchev ,
Victor-Valentin Hodirnau ,
Bettina Zens ,
Christoph Möhl ,
Frank Bradke  and
Florian Schur
Florian Fäßler
Affiliation:
Institute of Science and Technology Austria, Klosterneuburg, Niederosterreich, Austria
Georgi Dimchev
Affiliation:
Institute of Science and Technology Austria, Klosterneuburg, Niederosterreich, Austria
Victor-Valentin Hodirnau
Affiliation:
Institute of Science and Technology Austria, Klosterneuburg, Niederosterreich, Austria
Bettina Zens
Affiliation:
Institute of Science and Technology Austria, Klosterneuburg, Niederosterreich, Austria
Christoph Möhl
Affiliation:
German Center for Neurodegenerative Diseases (DZNE), Bonn, Nordrhein-Westfalen, Germany
Frank Bradke
Affiliation:
German Center for Neurodegenerative Diseases (DZNE), Bonn, Nordrhein-Westfalen, Germany
Florian Schur*
Affiliation:
Institute of Science and Technology Austria, Klosterneuburg, Niederosterreich, Austria
*
#Corresponding author

Abstract

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
The Promise of Cryo-Electron Tomography
Information
Microscopy and Microanalysis , Volume 26 , Supplement S2 , August 2020 , pp. 2518 - 2519
Check for updates
Copyright
Copyright © Microscopy Society of America 2020

References

Asano, S., Engel, B. D. & Baumeister, W. In Situ Cryo-Electron Tomography: A Post-Reductionist Approach to Structural Biology. J. Mol. Biol. 428, 332–343 (2016). doi:10.1016/J.JMB.2015.09.030CrossRefGoogle Scholar
Blanchoin, L., Boujemaa-Paterski, R., Sykes, C. & Plastino, J. Actin Dynamics, Architecture, and Mechanics in Cell Motility. Physiol. Rev. 94, 235–263 (2014). doi:10.1152/physrev.00018.2013CrossRefGoogle Scholar
Rottner, K. & Schaks, M. Assembling actin filaments for protrusion. Curr. Opin. Cell Biol. 56, 53–63 (2019). doi:10.1016/J.CEB.2018.09.004CrossRefGoogle Scholar
Winkler, C., Vinzenz, M., Small, J. V. & Schmeiser, C. Actin filament tracking in electron tomograms of negatively stained lamellipodia using the localized radon transform. J Struct Biol 178, 19–28 (2012). doi:http://dx.doi.org/10.1016/j.jsb.2012.02.011CrossRefGoogle Scholar