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Cryo-electron Tomography Workflows for Quantitative Analysis of Actin Networks Involved in Cell Migration

Published online by Cambridge University Press:  30 July 2020

Florian Fäßler ,
Georgi Dimchev ,
Victor-Valentin Hodirnau ,
Bettina Zens ,
Christoph Möhl ,
Frank Bradke  and
Florian Schur
Florian Fäßler
Affiliation:
Institute of Science and Technology Austria, Klosterneuburg, Niederosterreich, Austria
Georgi Dimchev
Affiliation:
Institute of Science and Technology Austria, Klosterneuburg, Niederosterreich, Austria
Victor-Valentin Hodirnau
Affiliation:
Institute of Science and Technology Austria, Klosterneuburg, Niederosterreich, Austria
Bettina Zens
Affiliation:
Institute of Science and Technology Austria, Klosterneuburg, Niederosterreich, Austria
Christoph Möhl
Affiliation:
German Center for Neurodegenerative Diseases (DZNE), Bonn, Nordrhein-Westfalen, Germany
Frank Bradke
Affiliation:
German Center for Neurodegenerative Diseases (DZNE), Bonn, Nordrhein-Westfalen, Germany
Florian Schur*
Affiliation:
Institute of Science and Technology Austria, Klosterneuburg, Niederosterreich, Austria
*
#Corresponding author

Abstract

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Type
The Promise of Cryo-Electron Tomography
Information
Microscopy and Microanalysis , Volume 26 , Supplement S2 , August 2020 , pp. 2518 - 2519
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Copyright
Copyright © Microscopy Society of America 2020

References

Asano, S., Engel, B. D. & Baumeister, W. In Situ Cryo-Electron Tomography: A Post-Reductionist Approach to Structural Biology. J. Mol. Biol. 428, 332–343 (2016). doi:10.1016/J.JMB.2015.09.030CrossRefGoogle Scholar
Blanchoin, L., Boujemaa-Paterski, R., Sykes, C. & Plastino, J. Actin Dynamics, Architecture, and Mechanics in Cell Motility. Physiol. Rev. 94, 235–263 (2014). doi:10.1152/physrev.00018.2013CrossRefGoogle Scholar
Rottner, K. & Schaks, M. Assembling actin filaments for protrusion. Curr. Opin. Cell Biol. 56, 53–63 (2019). doi:10.1016/J.CEB.2018.09.004CrossRefGoogle Scholar
Winkler, C., Vinzenz, M., Small, J. V. & Schmeiser, C. Actin filament tracking in electron tomograms of negatively stained lamellipodia using the localized radon transform. J Struct Biol 178, 19–28 (2012). doi:http://dx.doi.org/10.1016/j.jsb.2012.02.011CrossRefGoogle Scholar