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A Pre-Golgi Copartment Connecting Golgi Cisterns to the Perinuclear Space in Bovine Herpesvirus 1 (Bhv-1) Infected Mdbk Cells. Evidence for an Intracisternal Pathway

Published online by Cambridge University Press:  02 July 2020

P. Wild ,
E.M. Schraner ,
D. Cantieni ,
M. Engels ,
E. Loepfe ,
P. Walther  and
M. Müller
P. Wild
Affiliation:
Electron Microscopy, Institute of Veterinary Anatomy, Zürich
E.M. Schraner
Affiliation:
Electron Microscopy, Institute of Veterinary Anatomy, Zürich
D. Cantieni
Affiliation:
Electron Microscopy, Institute of Veterinary Anatomy, Zürich
M. Engels
Affiliation:
Institute of Virology, University of Ziirich, Zürich
E. Loepfe
Affiliation:
Institute of Virology, University of Ziirich, Zürich
P. Walther
Affiliation:
Electron Microscopic Unit, University of Ulm, Germany
M. Müller
Affiliation:
Laboratory for Electron Microscopy, Institute of Biochemistry, Swiss Federal Institute of Technology, Zürich, Switzerland
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Abstract

The nucleocapsid of herpesviruses is assembled within the nucleus and transported to the perinuclear space by budding through the inner nuclear membrane. The route from the perinuclear space to the plasma membrane for exocytotic release is assumed to involve loss of the acquired envelope by fusion with the outer nuclear membrane followed by wrapping of the nucleocapsid by Golgi membranes. Alternatively, virions are thought to leave the perinuclear space via vacuoles originating from the outer nuclear membrane. Non of these processes has been shown so far. We thus examined the nuclear periphery of MDBK cells infected with BHV-1 by cryobased electron microscopy. Infected cells were high-pressure frozen at 4, 5 and 6 hours of incubation, and freezesubstituted employing a protocol yielding high resolution of membranes.

Thin sections stained with uranyl acetate and lead citrate showed 3 distinct stages of viral envelopment at any time of incubation.

Type
Microbiology
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 738 - 739
Copyright
Copyright © Microscopy Society of America 2001

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References

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