Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-23T17:13:08.320Z Has data issue: false hasContentIssue false
  • English
  • Français

3d Image Reconstruction of Frog Virus 3 At 2.6nm

Published online by Cambridge University Press:  02 July 2020

X. Yan ,
V. Bowman ,
G. Murti ,
R. Goorha ,
A. Hyatt  and
T. S. Baker
X. Yan
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907
V. Bowman
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907
G. Murti
Affiliation:
Department of Virology and Molecular Biology, St. Jude Children's Hospital, Memphis, TN, 38105
R. Goorha
Affiliation:
Department of Virology and Molecular Biology, St. Jude Children's Hospital, Memphis, TN, 38105
A. Hyatt
Affiliation:
Australian Animal Health Laboratory, , Victoria, Australia
T. S. Baker
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907
Get access

Abstract

Frog virus 3 (FV3), a member of the genus Ranavirus (family Iridoviridae), was isolated from the leopard frog Rana pipiens. Members of this genus are recognized pathogens of amphibians, reptiles and fish and have been associated with declines of amphibians in the United Kingdom and the USA. Neutron-scattering studies showed that FV3 virions are composed of four concentric layers: an outer lipid envelope containing protein VP58; an icosahedral capsid shell with major capsid protein VP48; an inner lipid membrane with VP63 and 44; and a central core of dsDNA (∼170 kbps) and associated proteins. The FV3 genome has a terminally-redundant and circularly-permuted sequence. Naked virions (i.e. without the envelope lipid) contain more than 20 different proteins and about 9% lipid, which is required for infectivity. to date, structural information about FV3 has primarily been obtained in electron microscopy studies by means of thin-sectioning and negative staining techniques.

Type
Microbiology
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 746 - 747
Copyright
Copyright © Microscopy Society of America 2001

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Murti, K.G. et al., Advances in Virus Research. 30 (1985)1.CrossRefGoogle Scholar
2.Hyatt, A.D. et al., Archives of Virology. 145: 301–331.CrossRefGoogle Scholar
3.Chinchar, V. G. et al., Virology 137(1984)211.CrossRefGoogle Scholar
4.Goorha, R. and Murti, K. G., Proc. Natl. Acad. Sci. 79(1982)248.CrossRefGoogle Scholar
5.Willis, D. and Granoff, A.. Virology 61(1974)256.CrossRefGoogle Scholar
6.Adrian, M. et al., Nature 308(1984)32.CrossRefGoogle Scholar
7.Fuller, S. D. et al., J. Struct. Biology 116(1996)48.CrossRefGoogle Scholar
8.Yan, X. et al., Nature Structural Biology 7(2000)101.Google Scholar
9.Baker, T. S. and Cheng, R. H.. J. Struct. Biology 116(1996)120CrossRefGoogle Scholar
10.Toyoshima, C. et al, Ultramicrosc. 48(1993)165.CrossRefGoogle Scholar
11.We thank R. Ashmore for assistance with programming. Work supported in part by grants from theNIH (GM-33050), NSF (MCR-9527131), and a shared equipment grant from NSF (BIR 9112921) to T.S.BGoogle Scholar