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3DEM Data Collection of Cancer Biopsies: Comparing FIB-SEM and SBF-SEM

Published online by Cambridge University Press:  05 August 2019

Jessica L. Riesterer ,
Kevin Loftis ,
Kevin Stoltz ,
Erin Stempinski ,
Melissa Williams ,
Claudia S. López ,
Guillaume Thibault  and
Joe W. Gray
Jessica L. Riesterer*
Affiliation:
Oregon Health and Science University (OHSU), Center for Spatial Systems Biomedicine (OCSSB), Portland Oregon, USA. Multiscale Microscopy Core, OHSU, Portland Oregon, USA.
Kevin Loftis
Affiliation:
Oregon Health and Science University (OHSU), Center for Spatial Systems Biomedicine (OCSSB), Portland Oregon, USA.
Kevin Stoltz
Affiliation:
Oregon Health and Science University (OHSU), Center for Spatial Systems Biomedicine (OCSSB), Portland Oregon, USA.
Erin Stempinski
Affiliation:
Oregon Health and Science University (OHSU), Center for Spatial Systems Biomedicine (OCSSB), Portland Oregon, USA. Multiscale Microscopy Core, OHSU, Portland Oregon, USA.
Melissa Williams
Affiliation:
Oregon Health and Science University (OHSU), Center for Spatial Systems Biomedicine (OCSSB), Portland Oregon, USA. Multiscale Microscopy Core, OHSU, Portland Oregon, USA.
Claudia S. López
Affiliation:
Oregon Health and Science University (OHSU), Center for Spatial Systems Biomedicine (OCSSB), Portland Oregon, USA. Multiscale Microscopy Core, OHSU, Portland Oregon, USA. Pacific Northwest Center for CryoEM, Portland Oregon, USA.
Guillaume Thibault
Affiliation:
Oregon Health and Science University (OHSU), Center for Spatial Systems Biomedicine (OCSSB), Portland Oregon, USA.
Joe W. Gray
Affiliation:
Oregon Health and Science University (OHSU), Center for Spatial Systems Biomedicine (OCSSB), Portland Oregon, USA.
*
*Corresponding author: [email protected]

Abstract

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Type
Cutting-Edge Microscopy in the Pacific Northwest
Information
Microscopy and Microanalysis , Volume 25 , Supplement S2 , August 2019 , pp. 1194 - 1195
Copyright
Copyright © Microscopy Society of America 2019 

References

[1]Creech, M et al. , Scientific Reports [Online] 7, 40766 (2017), http://www.nature.com/articles/srep40766.Google Scholar
[2]Jorgens, D et al. , Journal of Cell Science 130 (2017), p. 177.Google Scholar
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[4]Peddie, C and Collinson, L, Micron 61 (2014), p. 9.Google Scholar
[5]Belevich, I et al. , PLoS Biology 14 (2016), p. e1002340. https://doi.org/10.1371/journal.pbio.1002340.Google Scholar
[6]Loftis, K et al. , Quantitative BioImaging (2019).Google Scholar
[7]Electron microscopy was performed at the Multiscale Microscopy Core (MMC) with technical support from the OHSU Center for Spatial Systems Biomedicine (OCSSB). The authors acknowledge funding from the OHSU Brenden-Colson Center for Pancreatic Care, the Prospect Creek Foundation and the OCSSB. Ms. Sharon Frase and Dr. Danielle Jorgens are thanked for discussions and contributions to this work.Google Scholar