Severe leaf blight of Japanese stiltgrass (JSG) from Bipolaris disease, causing significant decline in population density at some locations, has been reported sporadically in the field. Even so, much of the JSG in the mid-Atlantic is not diseased. Six populations of JSG from the field, one that was severely diseased by B. microstegii and the others “healthy,” were tested by artificial inoculation for susceptibility to both B. microstegii (five isolates) and B. drechsleri (three isolates). Populations of JSG in this study differed in their response to the two Bipolaris species, but within species of Bipolaris the plant responses were consistent. Plants from the diseased population of JSG from Frederick, MD, were very susceptible to B. microstegii, and plants from other populations from Maryland (three locations), Delaware, and Indiana were not. In contrast, B. drechsleri caused moderate disease on plants from all accessions but one, and it was significantly less aggressive than was B. microstegii on the susceptible accession of JSG. Results of a limited host range determination only with B. microstegii revealed hypersensitive responses, and therefore high levels of resistance, in corn (four cultivars) and sorghum (three accessions). The native, sympatric grass deertongue was not diseased in these tests. Results reveal a distinct differential response among populations of JSG to disease from B. microstegii, while in contrast, B. drechsleri is capable of causing disease on a broader range of JSG populations.

Classical biological control of waterhyacinth is difficult to evaluate against the backdrop of active herbicide programs. Two experiments evaluated the additive impact of herbivory by two biological control agents with three different rates of 2,4-D on waterhyacinth growth and development in outdoor concrete mesocosms. The herbicide 2,4-D was applied at three rates: (1) control (no herbicide), (2) reduced (2.1 kg ai ha−1), and (3) operational (4.3 kg ai ha−1). Biomass of waterhyacinth populations was reduced by 16.9% by biological control only, 10.5% by the reduced rate of herbicide alone, 44.6% by the operational rate, and 97.3% and 99.9% by the combination of biological control and the reduced and operational rates of herbicides, respectively. These results quantified the relative contributions of both tactics to waterhyacinth management and posit the question of whether further reductions in 2,4-D rates are possible without sacrificing efficacy.

Russian-olive is a nitrogen-fixing tree invading riparian corridors in western North America. The premise of revegetation after weed removal is that revegetation is required to return native species to a removal site and that revegetation improves site resistance to invasion or reinvasion via competitive exclusion. Therefore, we expected that revegetation would reduce invasive species cover and increase native species cover compared with non-revegetated controls. Native understory species diversity increased with time since removal. We recorded 18.2 native species in 2012, and 28.2 native species in 2016. Out of 22 planted species, 2 did not establish. Diversity in revegetated plots did not differ from unplanted controls, likely because species spread quickly across plot boundaries. Native perennial grass, seeded species, and annual bromes increased over time, while nonnative forbs and native forbs decreased over time. Only invasive perennial grass cover responded to the revegetation treatment with cover much higher in controls compared with revegetated plots (25.7% vs. 7.7%); this was likely a response to a preplanting herbicide treatment. All categories of species diversity except invasive species diversity increased over time. Only 4% of Russian-olive stumps resprouted in the first year of removal, less than 1% resprouted 2 yr after removal. There was no Russian-olive emergence from seed in the removal year, and seed emergence varied exponentially among following years. Seeded native species did not have trouble establishing once adequate spring moisture occurred in the second growing season after Russian-olive removal, indicating that removal did not present substantial obstacles to successful revegetation. Follow-up control of Russian-olive is critical after initial treatment.

In the city of Tromsø in northern Norway, invasive Tromsø palm (Norwegian: Tromsøpalme; English: Persian hogweed) is widespread. Although Tromsø palm has negative impacts on biodiversity and contains a phototoxic sap that burns human skin, it is also considered to be a local symbol of Tromsø city and is appreciated by many inhabitants. This study examined private landowners’ characteristics, perceptions, and landowners’ regulation of invasive Tromsø palm on their parcels on Tromsø Island in 2012 (vegetation season: May–September) to provide information concerning which landowner groups could be assisted by official regulation. Eleven key informants and 17 landowners were interviewed. Afterward, Tromsø palm on Tromsø Island was mapped using aerial photos and street-level photos from Google Maps®/Google Street View® and fieldwork verification. This distribution map was superimposed on a property map in a geographic information system to produce a map showing private parcels that contained Tromsø palm and associated neighboring parcels that did not contain Tromsø palm. Questionnaires were mailed to the 441 owners of the selected parcels, and 199 of the returned questionnaires were analyzed. Tromsø palm was more likely to be fully regulated/absent on a parcel that was inhabited (particularly if the owner lived on-site) and less likely to be fully regulated/absent if the parcel was jointly managed by several households. These findings indicate that authorities could focus their management efforts on supporting regulation efforts of those private landowners who own currently uninhabited or rented-out parcels and landowners of parcels jointly managed by several households. Furthermore, those landowners who found regulation measures against the plant on Tromsø Island important tended to have partly or fully regulated Tromsø palm on their plots. This might imply that information campaigns from authorities might encourage more landowners to regulate Tromsø palm.

Aphthona spp. flea beetles were released in two ecological sites of the Little Missouri National Grasslands in southwestern North Dakota in 1999 to control leafy spurge. The change in leafy spurge density and soil seedbank composition was monitored to evaluate the effectiveness of the biological weed control agent and the associated change in plant communities 5, 10, and 15 yr after release in loamy overflow (valleys) and loamy sites (ridges). In 2014, 15 yr after release, leafy spurge stem density had decreased 94% from 110 to 7 stems m−2 in the loamy overflow sites and 88% from 78 to 9 stems m−2 in the loamy sites. Leafy spurge represented only 2% and 6% of the loamy overflow and loamy seedbanks in 2004, respectively, compared with nearly 67% and 70%, respectively, in 1999. There was a slow shift to reintroduction of native species into the seedbank over the last 15 yr. The number of desirable species increased to 21 by 2014 (more than three times the number of species in 1999) in the loamy overflow sites, and doubled to 14 species in the loamy sites, while less desirable forb species doubled in both sites. Desirable grass species doubled in the loamy overflow sites by 2014 but remained unchanged in loamy sites. Aphthona spp. successfully controlled leafy spurge for more than 15 yr without any additional control methods or costs to land managers and resulted in the slow return of a subset of native species.

Japanese knotweed is an invasive plant that occurs along waterways, highways, abandoned agricultural land, and other disturbed areas. It reduces plant diversity and can increase shoreline erosion. An experiment was conducted in Bible Hill and Antigonish, NS, Canada to evaluate early POST aminopyralid at 120 g ae ha−1 and POST imazapyr applications at 720 g ae ha−1 at maximum shoot height, flowering, senescence, maximum height+flowering, maximum height+senescence, flowering+senescence, and maximum height+flowering+senescence. Early POST aminopyralid only provided 10% to 15% control at 52 wk after treatment (WAT) whereas 83% to 100% control occurred following imazapyr applications at all application timings. Percent control at 2, 4, and 8 WAT tended to be higher where imazapyr followed aminopyralid. By 52 WAT, equivalent damage ratings and reductions in stem density occurred at both sites in all plots where imazapyr was applied. The use of aminopyralid or multiple imazapyr applications provided no additional benefit over a single imazapyr application. We conclude that early POST aminopyralid suppresses knotweed growth, which should facilitate late-season imazapyr applications, especially in large stands.

Effective control measures are required for the invasive forage grass smooth brome in native prairie to maintain native prairie diversity and function. The objective of this study was to assess the long-term effectiveness of glyphosate as a control method for smooth brome and to evaluate the subsequent recovery of native prairie species at Kernen Prairie near Saskatoon, SK, Canada. In 1999 and 2000, a total of forty 6- to 8-m-diameter patches of smooth brome were spot sprayed with glyphosate; community composition in each patch was monitored for 17 yr. Following glyphosate application, the abundance of smooth brome decreased, and recovery of native species richness and the abundance of important native species, including plains rough fescue, was observed. In the long term however, the elimination of smooth brome created empty niche space ultimately occupied by other invasive species, particularly Kentucky bluegrass. The spot application of glyphosate is thus an effective control method for reducing smooth brome in native prairie; however, maintaining desirable native species composition in this system posttreatment depends on other factors, including the presence of additional invasive species that may move in after the elimination of smooth brome.

The giant reed, Arundo donax is one of the worst invasive alien species globally, including South Africa, where it invades riparian areas across the country. Biological control is being considered to address the invasive potential and negative impacts of the weed. This study investigated the phylogeography of A. donax to guide the biological control program. To determine plant haplotype and genetic diversity, three regions of the chloroplast were sequenced and three microsatellite markers were analyzed in 40 samples from across the plant’s distribution in South Africa. It was determined that all populations of A. donax in South Africa were haplotype M1, which is the most widely distributed haplotype worldwide, believed to originate from the Indus Valley, Asia. In addition, no genetic diversity was found, indicating that all the A. donax populations in South Africa are essentially one clone. The results indicate that suitable biological control agents are likely to be found in the ancient native range of haplotype M1. This research has contributed to the global understanding of the phylogeography of A. donax and will guide the biological control program in South Africa.

Although an increasing number of investigations have been made into the evolution of alien species once introduced, few studies have identified the invasion routes of these introduced species. Because multiple introductions are common in invasive species, failing to take into account the introduced lineages can be misleading when studying evolutionary change in alien species after they begin to extend their ranges. In Japan, diverse lineages of ryegrasses (Lolium spp.) were introduced as forage crops and contaminants in trading grain and have expanded to sandy coasts. We studied the expansion route of populations established along the coasts of three geographic regions within Japan by comparing variations in morphology and nuclear microsatellite and chloroplast DNA in the two habitats where ryegrasses were first introduced: croplands and international seaports. Chloroplast DNA haplotypes did not differ significantly among habitats and regions, but the coastal and seaport populations displayed similar microsatellite genetic compositions and morphological characteristics. Our results revealed that coastal populations originated from seaport populations derived from contaminants. Selective forces from the past, including domestication and naturalization, may have assisted the introduced lineages in colonizing new habitats.

Fig buttercup is a perennial herb native to Europe, temperate Asia, and northern Africa. In eastern North America, fig buttercup competes with native spring ephemerals, complicating control techniques. If chemical control could be shifted earlier in the year, the potential to negatively impact spring ephemerals would be reduced. We tested glyphosate applications on fig buttercup in northern Virginia under three early phenological phases (preflowering, early flowering, and 50% flowering) to assess the effectiveness of early-season treatment. Treating when approximately half of the plants in the population were in flower resulted in a 95% decline in fig buttercup. Treating when the first flower in the population had emerged resulted in a 90% decline. No later phenological phases were treated. Control of fig buttercup led to an increase in cover of Japanese stiltgrass, an invasive grass.

The grass species Spartina alterniflora (smooth cordgrass) is an invasive ecosystem engineer with serious ecological consequences to intertidal mudflats and saltmarshes. The largest infestation in North America was in Willapa Bay, WA, where it occupied ~3,600 solid ha, covering ~27,000 ha of intertidal habitat. An extensive effort by land managers to eradicate S. alterniflora has been in place for over two decades. This article discusses (1) the success of the control and issues that will need to be resolved in order to achieve eradication; (2) post-control succession of affected tidelands to native marsh species, and (3) the impact that S. alterniflora removal has had on shorebird usage of affected tidelands.

Aminocyclopyrachlor (AMCP) will control many invasive broadleaf weeds, but the susceptibility of desirable forbs is not widely known. Native prairie response to AMCP was evaluated near Fargo, ND, and Felton, MN, in the Northern Great Plains. Both sites had high floristic quality prior to treatment, with 33 and 80 different species at Fargo and Felton, respectively. AMCP was applied at 140 g ha−1 in July 2014 to coincide with leafy spurge and Canada thistle treatment timing. AMCP altered the plant communities and reduced foliar cover of undesirable species, high seral forbs (undisturbed stable communities), and low seral forbs (early succession in disturbed communities) at both locations at 10 and 14 mo after treatment (MAT). AMCP reduced Canada thistle and leafy spurge in Fargo and eliminated hedge bindweed, prickly lettuce, and black medic in Felton. High seral forb foliar cover was reduced at 10 and 14 MAT from 20% to 2% and 3% in Fargo and from 19% to 1.6% and 2% in Felton, respectively. The high seral forb species birdfoot violet, white panicled aster, northern bedstraw, Canada goldenrod, purple meadowrue, and American vetch were reduced at both locations. Low seral forb cover also decreased at 10 MAT from 22% to 10% in Fargo and from 12% to 1% in Felton, respectively. By 14 MAT, low seral species in Fargo recovered to 16%, but recovery was much slower in Felton and slightly increased to 1.5%. After treatment high and low seral monocot species increased at both sites, likely due to reduced competition from susceptible species. AMCP reduced richness, evenness, and diversity at both locations at 10 and 14 MAT; therefore, floristic quality declined. A decline in diversity is generally undesirable but could have beneficial effects if invasive weeds and other undesirable species are reduced or eliminated.

Woolly distaff thistle is a long-lived winter annual that threatens the ranching and dairy industries within the North Coast counties of California, particularly the organic producers. No peer-reviewed publications have documented effective control options or integrated management approaches for this species. We conducted two experiments, each replicated, in Marin County, California. The first compared several conventional herbicides at two timings and rates, while the second compared a conventional herbicide treatment with organic and integrated organic control methods, including an organic herbicide (mixture of capric and caprylic acids). Results of the conventional herbicide treatments showed most spring applications (March or April) of aminopyralid, aminocyclopyrachlor, clopyralid, and combinations of aminopyralid + triclopyr, or aminocyclopyrachlor + chlorsulfuron had greater than 99% control of woolly distaff thistle with fewer than 1.5 seedlings per 27-m2 plot by the end of the growing season. Higher rates were generally necessary to achieve the same level of control with winter (January) applications. In the organic herbicide treatments, the most consistent treatment was a combination of mowing followed by 9% (v/v) or the organic herbicide. This treatment was slightly less effective compared with aminopyralid but did have better than 95% control of woolly distaff thistle. The results of this study provide control options for both conventional and organic ranching practices where woolly distaff thistle is a problem.

Kentucky bluegrass (Poa pratensis L.) invades northern Great Plains rangelands. On the Sheyenne National Grassland in southeastern North Dakota, three research sites, each with a different level of Kentucky bluegrass invasion, were chosen to evaluate effectiveness of burning and burning–herbicide combinations to control Kentucky bluegrass. Initial Kentucky bluegrass invasion levels were 37%, 77%, and 91% for LOW, MODERATE, and HIGH invaded sites, respectively. Within each invaded site, four replicated strips (20 by 60 m) were established, with half of each strip burned in late October 2005 and the other half burned in early May 2006. Herbicide treatments of (1) no herbicide, (2) 2.24 kg ha−1 of glyphosate, and (3) 0.43 kg ha−1 of imazapic were randomly assigned to 10 by 20 m subplots within each burn. Control plots were established at the same time. Relative basal cover of native grass, native forb, and Kentucky bluegrass was estimated annually using 50 10-point frames within each subplot. On the HIGH site in 2006, fall-burned plots with a spring glyphosate application had three times the native grass cover and only one fourth of the Kentucky bluegrass cover compared with controls. Similar results with the same treatment occurred at the MODERATE site. Native grasses became the most abundant plant community on these plots in the MODERATE and HIGH sites within 1 yr. Treatment differences were transitory, and the LOW site differed from the MODERATE and HIGH sites. In 2007, on fall-burned plots with spring glyphosate application, the amount of Kentucky bluegrass was 14% and 30%, and native grass species were 52% and 42% on the MODERATE and HIGH sites, respectively, which was similar to the initial values on the LOW site. These data emphasize the importance of initial invasion level in developing restoration strategies and provide evidence burning and herbicide combinations can be valuable management tools even on heavily invaded grasslands.

Exotic weed propagules (seeds or fruits) often contaminate goods intended for import into Australia. Biosecurity officers must identify such propagules to manage risks, prevent incursions, and decide on potentially costly actions such as cleaning, treating, or destroying cargo. According to observations made by the Australian Department of Agriculture and Water Resources, more than 20% of the propagules found by biosecurity officers currently cannot be identified. By far the most important weed families contributing to seed load of imported goods are the grass family (Poaceae) and the daisy family (Asteraceae), accounting together for approximately 80% of cases. To facilitate fast and secure identification, increase the capabilities of biosecurity staff, and reduce the risk of weed incursions, we have developed an interactive digital identification key to the propagules of an initial priority list of 43 species of Asteraceae.

Interest exists in planting mixed forb–grass prairies in the midwestern United States. Aminopyralid or clopyralid can be used to suppress competition from invasive plants prior to seeding prairies. As these active ingredients are known to persist, concern exists that reductions in forb establishment could occur. We tested whether common midwestern forb species could tolerate an application of aminopyralid or clopyralid alone or in combination the summer prior to seeding, and whether fall dormant or spring seeding date influenced establishment. This experiment was performed in Beresford, SD, and Arlington, WI, where aminopyralid (54 or 123 g ae ha−1), clopyralid (237 and 420 g ae ha−1), or aminopyralid+clopyralid (54+237 g ae ha−1) were applied to a prepared seedbed in July of 2009. Ten forbs were seeded in November 2009 as a dormant seeding and in April 2010 as a spring seeding at both locations, and establishment was assessed 12 and 24 mo after treatment (MAT). Results were site and species specific. Time of seeding was an important driver of plant counts at both locations 12 and 24 MAT. In Wisconsin at 12 MAT, 60% of species studied exhibited higher counts in the spring seeding. This trend persisted in some, but was not consistent across all 10 species. In South Dakota, 80% of species studied had higher counts at 12 and 24 MAT, but differences were species specific and often differed from those studied in Wisconsin. Those species that had higher counts in spring seeding at 12 MAT, maintained higher counts at 24 MAT. Forbs planted in plots treated with herbicides did not differ from plots left untreated at either location. Results suggest native forbs typically seeded in the upper Midwest can tolerate these herbicides when applied at least 4 mo prior to seeding.