Hostname: page-component-848d4c4894-pjpqr Total loading time: 0 Render date: 2024-07-03T04:26:14.641Z Has data issue: false hasContentIssue false
  • English
  • Français

The replication of the chromosome of Pseudomonas aeruginosa strain 1: II. Sequential mutagenesis of synchronized cultures

Published online by Cambridge University Press:  14 April 2009

R. J. Booker  and
J. S. Loutit
R. J. Booker
Affiliation:
Department of Microbiology, University of Otago, Dunedin, New Zealand
J. S. Loutit
Affiliation:
Department of Microbiology, University of Otago, Dunedin, New Zealand

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The order of replication of a series of genes in Pseudomonas aeruginosa has been studied in synchronized cultures using a method based on the technique of sequential mutagenesis. This technique relies on the increased susceptibility of the replication point of the bacterial chromosome to mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine. The genes studied were those previously mapped by conjugation and whose order of replication had been studied by an investigation of gene frequencies in exponential populations. The results are consistent with the idea that there is two-way replication of the chromosome of P. aeruginosa starting at a point near trp-1 and arg-6. They also confirm that the two linkage groups which have been found by conjugation replicate at different times. If the assumption is made that there is only one chromosome in P. aeruginosa, the results can be used to show how the two linkage groups may possibly be joined together and the order is such that there would have to be two sites of attachment for the sex factor FP2.

Type
Research Article
Information
Genetics Research , Volume 25 , Issue 3 , June 1975 , pp. 215 - 227
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

Altenbern, R. A. (1971). Marker frequency analysis mapping of the Staphylococcus aureus chromosome. Canadian Journal of Microbiology 17, 903.Google Scholar
Bird, R. & Lark, K. G. (1968). Initiation and termination of DNA replication after amino acid starvation of E. coli 15T-. Cold Spring Harbour Symposium on Quantitative Biology 33, 799.Google Scholar
Booker, R. J. & Loutit, J. S. (1974). The order of replication of chromosomal markers in Pseudomonas aeruginosa strain 1. I. Marker frequency analysis by transduction. Genetical Research 23, 145.CrossRefGoogle ScholarPubMed
Cerda-Olmedo, E., Hanawalt, P. C. & Guerola, M. (1968). Mutagenesis of the replication point by nitrosoguanidine: Map and pattern of replication of the Escherichia coli chromosome. Journal of Molecular Biology 33, 705.CrossRefGoogle ScholarPubMed
Cooper, S. & Helmstetter, C. E. (1968). Chromosome replication and the division cycle of Escherichia coli B/r. Journal of Molecular Biology 31, 519.CrossRefGoogle ScholarPubMed
Giles, K. W. & Myers, A. (1965). An improved diphenylamine method for the estimation of DNA. Nature 206, 93.Google Scholar
Gomori, G. (1955). Preparation of buffers for use in enzyme studies. In Methods in Enzymology 1, 138. New York: Academic Press.CrossRefGoogle Scholar
Hohlfeld, R. & Vielmetter, W. (1973). Bidirectional growth of the Escherichia coli chromosome. Nature, New Biology 242, 130.Google Scholar
Lark, K. G. (1972). Evidence for the direct involvement of RNA in the initiation of DNA replication in Escherichia coli 15T-. Journal of Molecular Biology 64, 47.Google Scholar
Lark, K. G., Repko, T. & Hoffman, B. J. (1963). The effect of amino acid deprivation on subsequent DNA replication. Biochimica et Biophysica Acta 76, 9.CrossRefGoogle Scholar
Loutit, J. S. (1969). Investigation of the mating system of Pseudomonas aeruginosa strain 1. IV. Late markers. Genetical Research 13, 91.CrossRefGoogle Scholar
Loutit, J. S. & Marinus, M. G. (1968). Investigation of the mating system of Pseudomonas aeruginosa strain 1. H. Mapping of a number of early markets. Genetical Research 12, 37.CrossRefGoogle Scholar
Loutit, J. S., Pearce, L. E. & Marinus, M. G. (1968). Investigation of the mating system of Pseudomonas aeruginosa strain 1. I. Kinetic studies. Genetical Research 12, 129.Google ScholarPubMed
MaaløE, O. & Hanawalt, P. C. (1961). Thymine deficiency and the normal DNA replication cycle. Journal of Molecular Biology 3, 144.Google Scholar
Ward, C. B., Hane, M. W. & Glaser, D. A. (1970). Synchronous reinitiation of chromosome replication in E. coli B/r after nalidixic acid treatment. Proceedings of the National Academy of Sciences, U.S.A. 66, 365.CrossRefGoogle Scholar
Wolf, B., Pato, M. L., Ward, C. B. & Glaser, D. A. (1968). On the origin and direction of replication of the E. coli chromosome. Cold Spring Harbour Symposium on Quantitative Biology 33, 575.CrossRefGoogle ScholarPubMed