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A recombination test to classify mutants of Bacillus subtilis of identical phenotype

Published online by Cambridge University Press:  14 April 2009

A. Galizzi ,
A. G. Siccardi ,
G. Mazza ,
U. Canosi  and
M. Polsinelli
A. Galizzi
Affiliation:
Istituto di Genetica, Università di Pavia, and Laboratorio di Genetica Biochimica ed Evoluzionistica del Consiglio Nazionale delle Ricerche, Via S. Epifanio 14, 27100 Pavia, Italy
A. G. Siccardi
Affiliation:
Istituto di Genetica, Università di Pavia, and Laboratorio di Genetica Biochimica ed Evoluzionistica del Consiglio Nazionale delle Ricerche, Via S. Epifanio 14, 27100 Pavia, Italy
G. Mazza
Affiliation:
Istituto di Genetica, Università di Pavia, and Laboratorio di Genetica Biochimica ed Evoluzionistica del Consiglio Nazionale delle Ricerche, Via S. Epifanio 14, 27100 Pavia, Italy
U. Canosi
Affiliation:
Istituto di Genetica, Università di Pavia, and Laboratorio di Genetica Biochimica ed Evoluzionistica del Consiglio Nazionale delle Ricerche, Via S. Epifanio 14, 27100 Pavia, Italy
M. Polsinelli
Affiliation:
Istituto di Genetica, Università di Pavia, and Laboratorio di Genetica Biochimica ed Evoluzionistica del Consiglio Nazionale delle Ricerche, Via S. Epifanio 14, 27100 Pavia, Italy

Summary

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We have developed a recombination test in Bacillus subtilis that provides a tool for rapid genetic classification of mutants of identical phenotype. The test has been used to classify 25 ts mutants in nine recombination classes that have been proved by independent evidence to correspond to nine genetic loci.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 1 , February 1976 , pp. 47 - 58
Copyright
Copyright © Cambridge University Press 1976

References

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