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Manganese mutagenesis in yeast: II. Conditions of induction and characteristics of mitochondrial respiratory deficient Saccharomyces cerevisiae mutants induced with manganese and cobalt

Published online by Cambridge University Press:  14 April 2009

Wieslawa Prazmo ,
Ewa Balbin ,
Hanna Baranowska ,
Anna Ejchart  and
Aleksandra Putrament
Wieslawa Prazmo
Affiliation:
Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Rakowiecka 36, Warsaw, Poland
Ewa Balbin
Affiliation:
Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Rakowiecka 36, Warsaw, Poland
Hanna Baranowska
Affiliation:
Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Rakowiecka 36, Warsaw, Poland
Anna Ejchart
Affiliation:
Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Rakowiecka 36, Warsaw, Poland
Aleksandra Putrament
Affiliation:
Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Rakowiecka 36, Warsaw, Poland

Summary

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Manganese and cobalt are capable of inducing ρ− mutations* in non-growing cells of Saccharomyces cerevisiae, but their mutagenic action is much stronger in growing cells. At a given concentration cobalt and manganese can be either strongly mutagenic or non-mutagenic, depending on the cell density.

Most of the ρ− mutants induced with manganese and a considerable proportion of those induced with cobalt are suppressive and/or transmit drug resistance markers, so they must still carry mitochondrial DNA. Cobalt can decrease suppressiveness with low efficiency and eliminate drug resistance markers from established ρ− clones.

Type
Research Article
Information
Genetics Research , Volume 26 , Issue 1 , August 1975 , pp. 21 - 29
Copyright
Copyright © Cambridge University Press 1975

References

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