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Complementation of npf mutations in diploid amoebae of Physarum polycephalum: the basis for a general method of complementation analysis at the amoebal stage

Published online by Cambridge University Press:  14 April 2009

Roger W. Anderson  and
Philip J. Youngman
Roger W. Anderson
Affiliation:
Department of Biochemistry, University of Oxford, Oxford 0X1 3QU, England
Philip J. Youngman
Affiliation:
Department of Biology, Massachusetts, Institute of Technology, Cambridge, Massachusetts 02139, USA

Summary

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Haploid amoebae of Physarum polycephalum may form plasmodia by crossing, a sexual process that involves cellular and nuclear fusions, or by selfing, an asexual process in which the development of a single amoeba into a plasmodium may involve neither cellular nor nuclear fusion. Mutant strains (npf) in which selfing is suppressed were previously assigned to several functional groups on the basis of their ability to cross with one another in certain combinations. In the present study hybrid, diploid amoebae were isolated from both crossing-compatible and incompatible mixtures of npf mutants. The diploid amoebae from mixtures of compatible strains readily formed plasmodia by selfing, but selfing was suppressed in the diploids from incompatible mixtures. Thus the crossing tests between npf mutants may be viewed as complementation tests: their results reflect the differing selfing abilities of the hybrid, diploid amoebae that formed in each mixture. Genetical and environmental factors affecting the efficiency of formation of diploid amoebae were studied, and the diploids were shown to be stable during repeated subcultures. Although diploid amoebae carrying complementing npf mutations readily formed plasmodia by selfing at 26 δC, they could be cultured without plasmodium formation at 30 δC, a temperature that also inhibited selfing of the haploid npf+ strains. Ways are discussed of exploiting this combination of properties in a general procedure for isolating and testing diploids for dominance and complementation of amoebal mutations in P. polycephalum.

Type
Research Article
Information
Genetics Research , Volume 45 , Issue 1 , February 1985 , pp. 21 - 35
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Adler, P. N. (1975). Control of the differentiated state in Physarum polycephalum. Ph.D. Thesis, Massachusetts Institute of Technology.Google Scholar
Adler, P. N. & Holt, C. E. (1974). Genetic analysis in the Colonia strain of Physarum polycephalum: heterothallic strains that mate with and are partially isogenic to the Colonia strain. Genetics 78, 1051–1062.Google Scholar
Adler, P. N. & Holt, C. E. (1977). Mutations increasing asexual plasmodium formation in Physarum polycephalum. Genetics 87, 401–420.Google Scholar
Anderson, R. W. (1976). Analysis of the amoebal-plasmodial transition in Physarum polycephalum. Ph.D. Thesis, University of Leicester.Google Scholar
Anderson, R. W. (1977). A plasmodial colour mutation in the Myxomycete Physarum polycephalum. Genetical Research 30, 301–306.Google Scholar
Anderson, R. W. (1979). Complementation of amoebal-plasmodial transition mutants in Physarum polycephalum. Genetics 91, 409–419.Google Scholar
Anderson, R. W., Cooke, D. J. & Dee, J. (1976). Apogamic development of plasmodia in the Myxomycete Physarum polycephalum: A cinematographic analysis. Protoplasma 89, 29–40.CrossRefGoogle ScholarPubMed
Anderson, R. W. & Dee, J. (1977). Isolation and analysis of amoebal-plasmodial transition mutants in the Myxomycete Physarum polycephalum. Genetical Research 29, 21–34.Google Scholar
Anderson, R. W. & Holt, C. E. (1981). Revertants of selfing (gad) mutants in Physarum polycephalum. Developmental Genetics 2, 253–267.Google Scholar
Anderson, R. W. & Truitt, C. L. (1983). A new type of plasmodium formation in Physarum polycephalum. Genetical Research 42, 285–296.CrossRefGoogle Scholar
Collett, J. I., Holt, C. E. & Huttermann, A. (1983). Plasmodium formation in Physarum polycephalum: Cytological events and their timing relative to commitment. Cell Biology International Reports 7, 819–825.Google Scholar
Collins, O. R. & Tang, H. (1977). New mating types in Physarum polycephalum. Mycologia 69, 421–423.Google Scholar
Cooke, D. J. & Dee, J. (1975). Methods for the isolation and analysis of plasmodial mutants in Physarum polycephalum. Genetical Research 24, 175–187.CrossRefGoogle Scholar
Davidow, L. S. & Holt, C. E. (1977). Mutants with decreased differentiation to plasmodia in Physarum polycephalum. Molecular and General Genetics 155, 291–300.Google Scholar
Dee, J. (1966). Multiple alleles and other factors affecting plasmodium formation in the true slime mould Physarum polycephalum Schw. Journal of Protozoology 13, 610–616.Google Scholar
Dee, J. (1978). A gene unlinked to mating-type affecting crossing between strains of Physarum polycephalum. Genetical Research 31, 85–92.CrossRefGoogle Scholar
Dee, J. (1982). Genetics of Physarum polycephalum. In Cell biology of Physarum and Didymium, vol. 1: Organisms, Nucleus and Cell Cycle (ed. Aldrich, H. C. and Daniel, J. W.), pp. 211–251. New York: Academic Press.Google Scholar
Gorman, J. A., Dove, W. F. & Shaibe, E. (1979). Mutations affecting the initiation of plasmodial development in Physarum polycephalum. Developmental Genetics 1, 47–60.CrossRefGoogle Scholar
Honey, N. K., Poulter, R. T. M. & Aston, R. J. (1982). Non-selfing mutants from selfing (het −) strains of Physarum polycephalum. Genetical Research 39, 261–273.Google Scholar
Honey, N. K., Poulter, R. T. M. & Teale, D. M. (1979). Genetic regulation of differentiation in Physarum polycephalum. Genetical Research 34, 131–142.CrossRefGoogle Scholar
Honey, N. K., Poulter, R. T. M. & Winter, P. J. (1981). Selfing mutants from heterothallic strains of Physarum polycephalum. Genetical Research 37, 113–121.CrossRefGoogle Scholar
Kirouac-Brunet, J., Masson, S. & Pallotta, D. J. (1981). Multiple allelism at the matB locus in Physarum polycephalum. Canadian Journal of Genetics and Cytology 23, 9–16.Google Scholar
Poulter, R. T. M. & Dee, J. (1968). Segregation of factors controlling fusion between plasmodia of the true slime mould Physarum polycephalum. Genetical Research 12, 71–79.CrossRefGoogle ScholarPubMed
Sauer, H. W. (1982). Developmental biology of Physarum. Cambridge University Press.Google Scholar
Shinnick, T. M., Anderson, R. W. & Holt, C. E. (1983). Map and function of gad mutations in Physarum polycephalum. Genetical Research 42, 41–57.Google Scholar
Shinnick, T. M. & Holt, C. E. (1977). A mutation (gad) linked to mt and affecting asexual plasmodium formation in Physarum polycephalum. Journal of Bacteriology 131, 247–250.Google Scholar
Shinnick, T. M., Pallotta, D. J., Jones-Brown, Y. R., Youngman, P. J. & Holt, C. E. (1978). A gene, imz, affecting the pH sensitivity of zygote formation in Physarum polycephalum. Current Microbiology 1, 163–166.Google Scholar
Wheals, A. E.(1973). Developmental mutants in ahomothallic strain of Physarum polycephalum. Genetical Research 21, 79–86.Google Scholar
Youngman, P. J. (1979). Studies concerning the regulation of plasmodium formation in Physarum polycephalum and other Myxomycetes. Ph.D. Thesis, Massachusetts Institute of Technology.Google Scholar
Youngman, P. J., Adler, P. N., Shinnick, T. M. & Holt, C. E. (1977). An extracellular inducer of asexual plasmodium formation in Physarum polycephalum. Proceedings of the National Academy of Sciences (USA) 74, 1120–1124.CrossRefGoogle ScholarPubMed
Youngman, P. J., Anderson, R. W. & Holt, C. E. (1981). Two multiallelic mating compatibility loci separately regulate zygote formation and zygote differentiation in the Myxomycete Physarum polycephalum. Genetics 97, 513–530.CrossRefGoogle ScholarPubMed
Youngman, P. J., Pallotta, D. J., Hosler, B., Struhl, G. & Holt, C. E. (1979). A new mating compatibility locus in Physarum polycephalum. Genetics 91, 683–693.Google Scholar