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Assessment of Magnaporthe grisea mating type by PCR

Published online by Cambridge University Press:  12 February 2007

Wang Bao-Hua ,
Lu Guo-Dong ,
Li Hai-Ming ,
Lin Yan  and
Wang Zong-Hua
Wang Bao-Hua
Affiliation:
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Lu Guo-Dong*
Affiliation:
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Li Hai-Ming
Affiliation:
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Lin Yan
Affiliation:
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Wang Zong-Hua*
Affiliation:
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China
*
*Corresponding author: Email [email protected]; [email protected]
*Corresponding author: Email [email protected]; [email protected]
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Abstract

Analysis of mating type can provide an evaluation of the population genetic diversity of the rice blast fungus, Magnaporthe grisea. According to the sequences of MAT1-1 and MAT1-2 genes of the fungus, two pairs of PCR primers specific to the MAT1-1 and MAT1-2 alleles were designed, and the PCR thermal profile was also optimized. To confirm its application in mating type assessment, 10 tester isolates were tested by PCR. The PCR amplification pattern of these tester isolates corresponded to their known mating type. Furthermore, 150 rice-field isolates from Fujian Province were mated with tester isolates GUY11 and KA3 side by side and also tested by PCR. Results showed that 95.1% of 123 fertile isolates were the same in mating type as determined by both PCR-amplified allele-specific fragments and mating with GUY11/KA3. Among 27 sterile isolates determined by GUY11 and KA3, seven were MAT1-1 and 20 were MAT1-2 as determined by PCR. This study indicates that PCR is applicable in assessing M. grisea mating type and especially is capable of predicting the potential mating type of sterile isolates in the natural population of the fungus.

Keywords

Magnaporthe griseamating typePCR
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 1 , April 2005 , pp. 1 - 5
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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