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Successful development of microsatellite markers in a challenging species: the horizontal borer Austroplatypus incompertus (Coleoptera: Curculionidae)

Published online by Cambridge University Press:  11 April 2011

S. Smith ,
T. Joss  and
A. Stow
S. Smith*
Affiliation:
Department of Biological Sciences, Macquarie University, NSW, Australia, 2109
T. Joss
Affiliation:
Department of Biological Sciences, Macquarie University, NSW, Australia, 2109
A. Stow
Affiliation:
Department of Biological Sciences, Macquarie University, NSW, Australia, 2109
*
*Author for correspondence Fax: +61 (0) 2 9850 9395 E-mail: [email protected]
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Abstract

The analysis of microsatellite loci has allowed significant advances in evolutionary biology and pest management. However, until very recently, the potential benefits have been compromised by the high costs of developing these neutral markers. High-throughput sequencing provides a solution to this problem. We describe the development of 13 microsatellite markers for the eusocial ambrosia beetle, Austroplatypus incompertus, a significant pest of forests in southeast Australia. The frequency of microsatellite repeats in the genome of A. incompertus was determined to be low, and previous attempts at microsatellite isolation using a traditional genomic library were problematic. Here, we utilised two protocols, microsatellite-enriched genomic library construction and high-throughput 454 sequencing and characterised 13 loci which were polymorphic among 32 individuals. Numbers of alleles per locus ranged from 2 to 17, and observed and expected heterozygosities from 0.344 to 0.767 and from 0.507 to 0.860, respectively. These microsatellites have the resolution required to analyse fine-scale colony and population genetic structure. Our work demonstrates the utility of next-generation 454 sequencing as a method for rapid and cost-effective acquisition of microsatellites where other techniques have failed, or for taxa where marker development has historically been both complicated and expensive.

Keywords

ambrosia beetleeucalyptusmicrosatellitenext-generation sequencing
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 101 , Issue 5 , October 2011 , pp. 551 - 555
Copyright
Copyright © Cambridge University Press 2011

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