The ultrastructure of spermatozoon and sperm production were observed in wolffish (Anarhichas lupus), a species with internal fertilization, from a broodstock. The mature spermatozoon has an elongated head, eccentric flagellum, and comparatively well developed middle piece. Based on the observations of sperm cells with the optical microscope, the mean lengths of spermatozoon head and flagellum were 3.3 and 21.2 μm respectively. A high variability of sperm cell size was observed due to the presence of spermatids and degenerated spermatozoa in the ejaculate. In most males, sperm production was registered throughout the whole year with a peak in December and January. Sperm production decreased to the end of the breeding season. The difference in volumes of ejaculate and sperm concentrations between males kept at two light cycles (18D: 6L and 6D: 18L) was not significant. Features of wolffish sperm are compared with data reported in the literature on other internally and externally fertilizing fish.

The incorporation of an attractant mixture by substitution of an equivalent amount of basal dietary mixture was studied in terms of feed intake and growth performance in European seabass juveniles (mean initial weight : 17 g) fed plant-protein rich diets during 21 days. Three diets had as main protein sources; fish meal, or one of two soy protein concentrates (S and E). To the basal mixture of these last two diets, as well as to a corn-gluten based diet, an attractant amino acid mixture was added at a 2.5% level. Daily feed intake was measured throughout the experimental period. Feed intake and weight gain were highest in seabass fed the fish meal based diet. In those fed the soy protein concentrate diets, the addition of the attractant mixture improved growth significantly; voluntary feed intake and feed efficiency were also ameliorated in thesegroups. In terms of growth, feed intake and protein utilisation, lowest values were found in fish fed with the corn-gluten rich diet, supplemented with the amino acid mixture.

Short-term storage over several days as well as cryopreservation of turbot (Scophthalmus maximus) sperm were studied. Two extenders, Ringer 200 and artificial seminal liquid (ASL2), are suggested for semen collection in order to avoid the deleterious effect of urine contamination, and for the purpose of short-term storage between 0 and 15 °C. Oxygen atmosphere is not suitable for turbot sperm storage. Turbot spermatozoa undergo cryopreservation with a high rate of success especially in a sucrose solution with 10% dimethyl sulfoxide (DMSO) and 10% egg yolk.