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Chapter 37 - Cryopreservation of Post-compaction Embryos for IVF

from Section 7 - Embryo Cryopreservation

Published online by Cambridge University Press:  07 August 2023

By
Juergen Liebermann  and
Juan Sebastian Sanz
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Over the past 15 years, vitrification has become a well-established and globally used procedure that allows important expansion of therapeutic strategies when IVF is used to treat infertility. Vitrification of human blastocysts allows us to maximize the potential for conception and prevents wastage of embryos. This chapter is written in a kind of ’recipe’ style by revealing step-by-step the application of this vitrification technology for cryopreserving human blastocysts. Provided data demonstrate the simplicity but also the robustness of this technique for post-compaction human embryo cryopreservation.

Keywords

HSV straw, human blastocyst, cryopreservation, aseptic vitrification, embryo transfer, cryostorage
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 271 - 277
Publisher: Cambridge University Press
Print publication year: 2023

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