Book contents
- Manual of Sperm Function Testing in Human Assisted Reproduction
- Cambridge Laboratory Manuals in Assisted Reproductive Technology
- Manual of Sperm Function Testing in Human Assisted Reproduction
- Copyright page
- Dedication
- Contents
- Contributors
- Short Biography
- Foreword
- Preface
- Introduction
- Chapter 1 Standard Semen Examination: Manual Semen Analysis
- Chapter 2 Standard Semen Analysis: Computer-Assisted Semen Analysis
- Chapter 3 Standard Semen Analysis: Home Sperm Testing
- Chapter 4 Standard Semen Analysis: Leukocytospermia
- Chapter 5 Standard Semen Analysis: Morphology
- Chapter 6 Sperm Vitality: Eosin-Nigrosin Dye Exclusion
- Chapter 7 Sperm Vitality: Hypo-Osmotic Swelling Test
- Chapter 8 Determination of Mitochondrial Membrane Potential by Flow Cytometry in Human Sperm Cells
- Chapter 9 Capacitation and Acrosome Reaction: Fluorescence Techniques to Determine Acrosome Reaction
- Chapter 10 Capacitation and Acrosome Reaction: Histochemical Techniques to Determine Acrosome Reaction
- Chapter 11 Zona Binding: Competitive Sperm-Binding Assay
- Chapter 12 Zona Binding: Hemizona Assay
- Chapter 13 Oolemma Binding: Sperm Penetration Assay
- Chapter 14 Oxidative Stress Testing: Direct Tests
- Chapter 15 Oxidative Stress Testing: Indirect Tests
- Chapter 16 Chromatin Condensation: Aniline Blue Stain
- Chapter 17 Chromatin Condensation: Chromomycin A3 (CMA3) Stain
- Chapter 18 Sperm Chromatin Structure: Toluidine Blue Staining
- Chapter 19 DNA Damage: TdT-Mediated dUTP Nick-End-Labelling Assay
- Chapter 20 DNA Damage: Sperm Chromatin Structure Assay
- Chapter 21 DNA Damage: COMET Assay
- Chapter 22 DNA Damage: Halo Sperm Test
- Chapter 23 DNA Damage: Fluorescent In-Situ Hybridization
- Chapter 24 Clinical Value of Sperm Function Tests
- Chapter 25 Future Developments: Sperm Proteomics
- Conclusion
- Index
- References
Chapter 21 - DNA Damage: COMET Assay
Published online by Cambridge University Press: 05 April 2021
- Manual of Sperm Function Testing in Human Assisted Reproduction
- Cambridge Laboratory Manuals in Assisted Reproductive Technology
- Manual of Sperm Function Testing in Human Assisted Reproduction
- Copyright page
- Dedication
- Contents
- Contributors
- Short Biography
- Foreword
- Preface
- Introduction
- Chapter 1 Standard Semen Examination: Manual Semen Analysis
- Chapter 2 Standard Semen Analysis: Computer-Assisted Semen Analysis
- Chapter 3 Standard Semen Analysis: Home Sperm Testing
- Chapter 4 Standard Semen Analysis: Leukocytospermia
- Chapter 5 Standard Semen Analysis: Morphology
- Chapter 6 Sperm Vitality: Eosin-Nigrosin Dye Exclusion
- Chapter 7 Sperm Vitality: Hypo-Osmotic Swelling Test
- Chapter 8 Determination of Mitochondrial Membrane Potential by Flow Cytometry in Human Sperm Cells
- Chapter 9 Capacitation and Acrosome Reaction: Fluorescence Techniques to Determine Acrosome Reaction
- Chapter 10 Capacitation and Acrosome Reaction: Histochemical Techniques to Determine Acrosome Reaction
- Chapter 11 Zona Binding: Competitive Sperm-Binding Assay
- Chapter 12 Zona Binding: Hemizona Assay
- Chapter 13 Oolemma Binding: Sperm Penetration Assay
- Chapter 14 Oxidative Stress Testing: Direct Tests
- Chapter 15 Oxidative Stress Testing: Indirect Tests
- Chapter 16 Chromatin Condensation: Aniline Blue Stain
- Chapter 17 Chromatin Condensation: Chromomycin A3 (CMA3) Stain
- Chapter 18 Sperm Chromatin Structure: Toluidine Blue Staining
- Chapter 19 DNA Damage: TdT-Mediated dUTP Nick-End-Labelling Assay
- Chapter 20 DNA Damage: Sperm Chromatin Structure Assay
- Chapter 21 DNA Damage: COMET Assay
- Chapter 22 DNA Damage: Halo Sperm Test
- Chapter 23 DNA Damage: Fluorescent In-Situ Hybridization
- Chapter 24 Clinical Value of Sperm Function Tests
- Chapter 25 Future Developments: Sperm Proteomics
- Conclusion
- Index
- References
Summary
Sperm are highly specialized cells, evolved to function as vehicles for the transport of the paternal genome to the oocyte. The sperm cell is characterized by a distinct head, mid-piece and tail, structured for a streamlined function. The sperm head consists of the haploid paternal genome (23 chromosomes), packed in a specific tight manner with the help of specialized proteins called protamines. The mid-piece consists of the centrosome and mitochondria, organelles that provide energy for sperm propulsion from the tail. The unique sperm structure, complimented with its motility, helps the sperm to swim through the male and female reproductive tract and penetrate the egg. Therefore, the primary function of the sperm is to successfully deliver the paternal genome to the oocyte.
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- Manual of Sperm Function Testing in Human Assisted Reproduction , pp. 202 - 212Publisher: Cambridge University PressPrint publication year: 2021