Dahan, MH, Tan, SL, Chung, J, Son, WY Clinical definition paper on in vitro maturation of human oocytes. Hum Reprod. 2016;31(7): 1383–6.CrossRefGoogle ScholarPubMed

Walls, ML, Hunter, T, Ryan, JP et al. In vitro maturation as an alternative to standard in vitro fertilization for patients diagnosed with polycystic ovaries: a comparative analysis of fresh, frozen and cumulative cycle outcomes. Hum Reprod. 2015;30(1):88–96.Google Scholar

Pincus, G, Enzmann, EV The comparative behavior of mammalian eggs in vivo and in vitro. The Journal of Experimental Medicine. 1935;62(5): 665–75.CrossRefGoogle ScholarPubMed

Edwards, RG Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes. Nature. 1965;208(5008): 349–51.Google Scholar

Cha, KY, Koo, JJ, Ko, JJ et al. Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertility and Sterility. 1991;55(1):109.Google Scholar

Trounson, A, Wood, C, Kausche, A. In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertility and Sterility. 1994;62(2):353.Google Scholar

De Vos, M, Ortega-Hrepich, C, Albuz, FK et al. Clinical outcome of non-hCG-primed oocyte in vitro maturation treatment in patients with polycystic ovaries and polycystic ovary syndrome. Fertil Steril. 2011;96(4): 860–4.Google Scholar

Chian, RC, Buckett, WM, Too, LL, Tan, SL Pregnancies resulting from in vitro matured oocytes retrieved from patients with polycystic ovary syndrome after priming with human chorionic gonadotropin. Fertil Steril. 1999;72(4): 639–42.Google Scholar

Junk, SM, Yeap, D. Improved implantation and ongoing pregnancy rates after single-embryo transfer with an optimized protocol for in vitro oocyte maturation in women with polycystic ovaries and polycystic ovary syndrome. Fertil Steril. 2012;98(4): 888–92.Google Scholar

Walls, ML, Douglas, K, Ryan, JP, Tan, J, Hart, R. In-vitro maturation and cryopreservation of oocytes at the time of oophorectomy. Gynecologic Oncology Reports. 2015;13:79–81.Google Scholar

Uzelac, PS, Delaney, AA, Christensen, GL, Bohler, HC, Nakajima, ST. Live birth following in vitro maturation of oocytes retrieved from extracorporeal ovarian tissue aspiration and embryo cryopreservation for 5 years. Fertil Steril. 2015;104(5): 1258–60.CrossRefGoogle ScholarPubMed

Segers, I, Mateizel, I, Van Moer, E et al. In vitro maturation (IVM) of oocytes recovered from ovariectomy specimens in the laboratory: a promising “ex vivo” method of oocyte cryopreservation resulting in the first report of an ongoing pregnancy in Europe. J Assist Reprod Genet. 2015;32(8): 1221–31.CrossRefGoogle ScholarPubMed

Ross, C TK, Child, T, Davies, J, Becker, C, Fatum, M. Immature oocyte retrieval followed by in vitro maturation (IVM) and vitrification in combination with laparoscopic ovarian tissue cryopreservation (OTCP) for fertility preservation: UK pilot study. Human Reproduction. 2016; 31(Supplement 1):i335.Google Scholar

Tang, H, Hunter, T, Hu, Y et al. Cabergoline for preventing ovarian hyperstimulation syndrome. Cochrane Database Syst Rev. 2012(2):CD008605.Google Scholar

Walls, ML, Ryan, JP, Keelan, JA, Hart, R. In vitro maturation is associated with increased early embryo arrest without impairing morphokinetic development of useable embryos progressing to blastocysts. Hum Reprod. 2015;30(8): 1842–9.CrossRefGoogle ScholarPubMed

Radesic, B, Tremellen, K. Oocyte maturation employing a GnRH agonist in combination with low-dose hCG luteal rescue minimizes the severity of ovarian hyperstimulation syndrome while maintaining excellent pregnancy rates. Hum Reprod. 2011;26(12): 3437–42.CrossRefGoogle ScholarPubMed

Boothroyd, C, Karia, S, Andreadis, N et al.Consensus statement on prevention and detection of ovarian hyperstimulation syndrome. Aust N Z J Obstet Gynaecol. 2015;55(6): 523–34.CrossRefGoogle ScholarPubMed

Oktay, K, Buyuk, E, Libertella, N, Akar, M, Rosenwaks, Z. Fertility preservation in breast cancer patients: a prospective controlled comparison of ovarian stimulation with tamoxifen and letrozole for embryo cryopreservation. J Clin Oncol. 2005;23(19): 4347–53.Google Scholar

Stopp, D, Cobo, A, Silber, S. Fertility preservation for age-related fertility decline. whilst maintaining the integrity of the oocyte. Lancet. 2014 October 4;384(9950): 1311–9.Google Scholar

Benard, J, Calvo, J, Comtet, M et al. Fertility preservation in women of the childbearing age: Indications and strategies. J Gynecol Obstet Biol Reprod (Paris). 2016;45(5): 424–44.Google ScholarPubMed

Huang, JY, Tulandi, T, Holzer, H et al. Cryopreservation of ovarian tissue and in vitro matured oocytes in a female with mosaic Turner syndrome: Case Report. Hum Reprod. 2008;23(2): 336–9.Google Scholar

van Erven, B, Gubbels, CS, van Golde, RJ et al. Fertility preservation in female classic galactosemia patients. Orphanet Journal of Rare Diseases. 2013;8:107.CrossRefGoogle ScholarPubMed

Prasath, EB, Chan, ML, Wong, WH et al. First pregnancy and live birth resulting from cryopreserved embryos obtained from in vitro matured oocytes after oophorectomy in an ovarian cancer patient. Hum Reprod. 2014;29(2): 276–8.Google Scholar

Mikkelsen, AL, Smith, SD, Lindenberg, S. In-vitro maturation of human oocytes from regularly menstruating women may be successful without follicle stimulating hormone priming. Human Reproduction. 1999;14(7): 1847–51.Google Scholar

Mikkelsen, A, Lindenberg, S. Benefit of FSH priming of women with PCOS to the in vitro maturation procedure and the outcome: a randomized prospective study. Reproduction. 2001;122(4): 587–92.Google Scholar

Junk, SM, Yeap, D. Improved implantation and ongoing pregnancy rates after single-embryo transfer with an optimized protocol for in vitro oocyte maturation in women with polycystic ovaries and polycystic ovary syndrome. Fertility and Sterility. 2012;98(4): 888–92.Google Scholar

Zheng, X, Wang, L, Zhen, X et al. Effect of hCG priming on embryonic development of immature oocytes collected from unstimulated women with polycystic ovarian syndrome. Reprod Biol Endocrinol. 2012;10:40.Google Scholar

Steptoe, PC, Edwards, RG Birth after the reimplantation of a human embryo. Lancet. 1978;12;2:366.Google Scholar

Sermon, K, Capalbo, A, Cohen, J et al. The why, the how and the when of PGS 2.0: current practices and expert opinions of fertility specialists, molecular biologists, and embryologists. Mol Hum Reprod. 2016;22: 845–57.CrossRefGoogle ScholarPubMed

Palermo, G, Joris, H, Devroey, P, Van Steirteghem, AC Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992;340:17–18.Google Scholar

Trounson, A, Mohr, L. Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo. Nature. 1983;305: 707–09.Google Scholar

Chen, C. Pregnancy after human oocyte cryopreservation. Lancet. 1986;1: 884–86.Google Scholar

Bunge, RG, Sherman, JK Fertilizing capacity of frozen human spermatozoa. Nature. 1953;172: 767–68.Google Scholar

Szeptycki, J, Bentov, Y. Cryopreservation of embryos and gametes: past, present, and future. In: Marco-Jimenez editor. Cryopreservation in Eukaryotes. IntechOpen, 2016.Google Scholar

Rienzi, L, Gracia, C, Maggiulli, R et al. Oocyte, embryo and blastocyst cryopreservation in ART: systematic review and meta-analysis comparing slow-freezing versus vitrification to produce evidence for the development of global guidance. Hum Reprod Update. 2017;23: 139–55.Google Scholar

Levi Setti, PE, Porcu, E, Patrizio, P et al. Human oocyte cryopreservation with slow freezing versus vitrification. Results from the National Italian Registry data, 2007–2011. Fertil Steril. 2014;102:90–95.CrossRefGoogle ScholarPubMed

Cobo, A, Garcia-Velasco, JA, Coello, A et al. Oocyte vitrification as an efficient option for elective fertility preservation. Fertil Steril. 2016;105: 755–64.CrossRefGoogle ScholarPubMed

Gardner, DK Textbook of Assisted Reproductive Technologies: Laboratory and Clinical Perspectives. 3rd edn. London: Informa Healthcare; 2009.Google Scholar

Engmann, L, Benadiva, C, Humaidan, P. GnRH agonist trigger for the induction of oocyte maturation in GnRH antagonist IVF cycles: a SWOT analysis. Reprod Biomed Online. 2016;32: 274–85.Google Scholar

Cakmak, H, Rosen, MP Random-start ovarian stimulation in patients with cancer. Curr Opin Obstet Gynecol. 2015;27: 215–21.Google Scholar

Nagy, ZP, Anderson, RE, Feinberg, EC, Hayward, B, Mahony, MC The human oocyte preservation experience (HOPE) registry: evaluation of cryopreservation techniques and oocyte source on outcomes. Reprod Biol Endocrinol. 2017;15:10.Google Scholar

Doyle, JO, Richter, KS, Lim, J et al. Successful elective and medically indicated oocyte vitrification and warming for autologous in vitro fertilization, with predicted birth probabilities for fertility preservation according to number of cryopreserved oocytes and age at retrieval. Fertil Steril. 2016;105: 459–66.CrossRefGoogle ScholarPubMed

Herrero, L, Pareja, S, Aragones, M et al. Oocyte versus embryo vitrification for delayed embryo transfer: an observational study. Reprod Biomed Online. 2014;29: 567–72.Google Scholar

Chang, CC, Elliott, TA, Wright, G et al. Prospective controlled study to evaluate laboratory and clinical outcomes of oocyte vitrification obtained in in vitro fertilization patients aged 30 to 39 years. Fertil Steril. 2013;99: 1891–97.Google Scholar

Cil, AP, Bang, H, Oktay, K. Age-specific probability of live birth with oocyte cryopreservation: an individual patient data meta-analysis. Fertility and Sterility; 2013;100: 492–99.Google Scholar

Cobo, A, Garcia-Velasco, JA, Domingo, J, Remohi, J, Pellicer, A. Is vitrification of oocytes useful for fertility preservation for age-related fertility decline and in cancer patients? Fertil Steril. 2013;99: 1485–95.Google Scholar

Garcia-Velasco, JA, Domingo, J, Cobo, A et al. Five years’ experience using oocyte vitrification to preserve fertility for medical and nonmedical indications. Fertil Steril. 2013;99: 1994–99.CrossRefGoogle ScholarPubMed

Nagy, ZP, Chang, CC, Shapiro, DB et al. Clinical evaluation of the efficiency of an oocyte donation program using egg cryo-banking. Fertil. Steril. 2009;92: 520–26.Google Scholar

Cobo, A, Meseguer, M, Remohi, J, Pellicer, A. Use of cryo-banked oocytes in an ovum donation programme: a prospective, randomized, controlled, clinical trial. Hum Reprod. 2010;25: 2239–46.CrossRefGoogle Scholar

Cobo, A, Remohi, J, Chang, CC, Nagy, ZP Oocyte cryopreservation for donor egg banking. Reprod Biomed Online. 2011;23: 341–46.Google Scholar

Cobo, A, Castellò, D, Vallejo, B et al. Outcome of cryotransfer of embryos developed from vitrified oocytes: double vitrification has no impact on delivery rates. Fertility and Sterility. 2013;99: 1623–30.Google Scholar

Dominguez, F, Castello, D, Remohi, J, Simon, C, Cobo, A. Effect of vitrification on human oocytes: a metabolic profiling study. Fertil Steril. 2013;99: 565–72.Google Scholar

Di Pietro, C, Vento, M, Guglielmino, MR et al. Molecular profiling of human oocytes after vitrification strongly suggests that they are biologically comparable with freshly isolated gametes. Fertil Steril. 2010;94: 2804–07.Google Scholar

Forman, EJ, Li, X, Ferry, KM et al. Oocyte vitrification does not increase the risk of embryonic aneuploidy or diminish the implantation potential of blastocysts created after intracytoplasmic sperm injection: a novel, paired randomized controlled trial using DNA fingerprinting. Fertil Steril. 2012;98: 644–49.Google Scholar

Cobo, A, Serra, V, Garrido, N et al. Obstetric and perinatal outcome of babies born from vitrified oocytes. Fertil Steril. 2014;102: 1006–15.CrossRefGoogle ScholarPubMed

Chian, RC, Huang, JY, Tan, SL et al. Obstetric and perinatal outcome in 200 infants conceived from vitrified oocytes. Reprod Biomed Online. 2008;16: 608–10.Google Scholar

Flaherty, SP, Payne, D, Swann, NJ, Mattews, CD. Aetiology of failed and abnormal fertilization after intracytoplasmic sperm injection. Hum Reprod 1995;10: 2623–29.Google Scholar

Flaherty, SP, Payne, D, Swann, NJ, Matthews, CD. Assessment of fertilization failure and abnormal fertilization after intracytoplasmic sperm injection (ICSI). Reprod Fertil Dev 1995;7:197–210.Google Scholar

Mahutte, NG, Arici, A. Failed fertilization: is it predictable? Curr Opin Obstet Gynecol 2003;15: 211–18.CrossRefGoogle ScholarPubMed

Sathananthan, AH. Paternal centrosomal dynamics in early human development and infertility. J Assist Reprod Genet 1998;15: 129–39.Google Scholar

Terada, Y, Nakamura, S, Simerly, C et al. Centrosomal function assessment in human sperm using heterologous ICSI with rabbit eggs: a new male factor infertility assay. Mol Reprod Dev 2004;67: 360–65.Google Scholar

Gupta, SK. Role of zona pellucida glycoproteins during fertilization in humans. J Reprod Immunol 2015;108:90–97.CrossRefGoogle ScholarPubMed

Hook, EB. Estimates of maternal age-specific risks of Down-syndrome birth in women aged 34–41. Lancet 7–3-1976;2: 33–34.CrossRefGoogle ScholarPubMed

Speroff, L. The effect of aging on fertility. Curr Opin Obstet Gynecol 1994;6: 115–20.CrossRefGoogle ScholarPubMed

Boulet, SL, Mehta, A, Kissin, DM et al. Trends in use of and reproductive outcomes associated with intracytoplasmic sperm injection. JAMA 2015;313: 255–63.Google Scholar

Center of Disease Control 2014. Assisted reproduction technology national summary report. 2017 page 5. https://www.cdc.gov/art/pdf/2014-report/art-2014-national-summary-report.pdf accessed September 14, 2018.Google Scholar

Dyer, S, Chambers, GM, de MJ et al. International committee for monitoring assisted reproductive technologies world report: Assisted reproductive technology 2008, 2009 and 2010. Hum Reprod 2016;31: 1588–609.Google Scholar

Russell, DL, Salustri, A. Extracellular matrix of the cumulus-oocyte complex. Semin Reprod Med 2006;24: 217–27.CrossRefGoogle ScholarPubMed

Yerushalmi, GM, Salmon-Divon, M, Yung, Y et al. Characterization of the human cumulus cell transcriptome during final follicular maturation and ovulation. Mol Hum Reprod 2014;20: 719–35.Google Scholar

Plachot, M, Junca, AM, Mandelbaum, J et al. Timing of in-vitro fertilization of cumulus-free and cumulus-enclosed human oocytes. Hum Reprod 1986;1: 237–42.Google Scholar

Avella, MA, Xiong, B, Dean, J. The molecular basis of gamete recognition in mice and humans. Mol Hum Reprod 2013;19: 279–89.Google Scholar

Avella, MA, Baibakov, B, Dean, J. A single domain of the ZP2 zona pellucida protein mediates gamete recognition in mice and humans. J Cell Biol 6–23-2014;205: 801–09.Google Scholar

Burkart, AD, Xiong, B, Baibakov, B, Jimenez-Movilla, M, Dean, J. Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy. J Cell Biol 4–2-2012;197:37–44.Google Scholar

Liu, DY, Garrett, C, Baker, HW. Clinical application of sperm-oocyte interaction tests in in vitro fertilization–embryo transfer and intracytoplasmic sperm injection programs. Fertil Steril 2004;82: 1251–63.Google Scholar

Sanders, JR, Swann, K. Molecular triggers of egg activation at fertilization in mammals. Reproduction 2016;152: R41–R50.Google Scholar

Williams, HL, Mansell, S, Alasmari, W et al. Specific loss of CatSper function is sufficient to compromise fertilizing capacity of human spermatozoa. Hum Reprod 2015;30: 2737–46.Google Scholar

Sun, QY, Schatten, H. Regulation of dynamic events by microfilaments during oocyte maturation and fertilization. Reproduction 2006;131:193–205.CrossRefGoogle ScholarPubMed

Capmany, G, Taylor, A, Braude, PR, Bolton, VN. The timing of pronuclear formation, DNA synthesis and cleavage in the human 1-cell embryo. Mol Hum Reprod 1996;2:299–306.Google Scholar

Aguilar, J, Motato, Y, Escriba, MJ et al. The human first cell cycle: impact on implantation. Reprod Biomed Online 2014;28: 475–84.Google Scholar

Sakkas, D, Urner, F, Bianchi, PG,et al. Sperm chromatin anomalies can influence decondensation after intracytoplasmic sperm injection. Hum Reprod 1996;11: 837–43.Google Scholar

Kvist, U. Sperm nuclear chromatin decondensation ability. An in vitro study on ejaculated human spermatozoa. Acta Physiol Scand Suppl 1980;486:1–24.Google Scholar

Esterhuizen, AD, Franken, DR, Becker, PJ et al. Defective sperm decondensation: a cause for fertilization failure. Andrologia 2002;34:1–7.Google Scholar

Collins, JA, Barnhart, KT, Schlegel, PN. Do sperm DNA integrity tests predict pregnancy with in vitro fertilization? Fertil Steril 2008;89: 823–31.Google Scholar

Sakkas, D, Alvarez, JG. Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis. Fertil Steril 3–1-2010;93: 1027–36.Google Scholar

Henkel, R. Sperm preparation: state-of-the-art–physiological aspects and application of advanced sperm preparation methods. Asian J Androl 2012;14: 260–69.Google Scholar

Huszar, G, Jakab, A, Sakkas, D et al. Fertility testing and ICSI sperm selection by hyaluronic acid binding: clinical and genetic aspects. Reprod Biomed Online 2007;14: 650–63.Google Scholar

Choe, SA, Tae, JC, Shin, MY et al. Application of sperm selection using hyaluronic acid binding in intracytoplasmic sperm injection cycles: a sibling oocyte study. J Korean Med Sci 2012;27: 1569–73.CrossRefGoogle ScholarPubMed

Brown, SG, Publicover, SJ, Mansell, SA et al. Depolarization of sperm membrane potential is a common feature of men with subfertility and is associated with low fertilization rate at IVF. Hum Reprod 2016;31: 1147–57.CrossRefGoogle ScholarPubMed

Kashir, J, Heindryckx, B, Jones, C et al. Oocyte activation, phospholipase C zeta and human infertility. Hum Reprod Update 2010;16:690–703.Google Scholar

Sfontouris, IA, Nastri, CO, Lima, ML et al. Artificial oocyte activation to improve reproductive outcomes in women with previous fertilization failure: a systematic review and meta-analysis of RCTs. Hum Reprod 2015;30: 1831–41.Google Scholar

Ebner, T, Montag, M, Montag, M et al. Live birth after artificial oocyte activation using a ready-to-use ionophore: a prospective multicentre study. Reprod Biomed Online 2015;30: 359–65.Google Scholar

Sfontouris, IA, Nastri, CO, Lima, ML et al. Artificial oocyte activation to improve reproductive outcomes in women with previous fertilization failure: a systematic review and meta-analysis of RCTs. Hum Reprod 2015;30: 1831–41.Google Scholar

Aydinuraz, B, Dirican, EK, Olgan, S, Aksunger, O, Erturk, OK. Artificial oocyte activation after intracytoplasmic morphologically selected sperm injection: A prospective randomized sibling oocyte study. Hum Fertil (Camb) 2016;19: 282–88.Google Scholar

Miller, N, Biron-Shental, T, Sukenik-Halevy, R et al. Oocyte activation by calcium ionophore and congenital birth defects: a retrospective cohort study. Fertil Steril 9–1-2016;106: 590–96.Google Scholar

Tavalaee, M, Kiani-Esfahani, A, Nasr-Esfahani, MH. Relationship between potential sperm factors involved in oocyte activation and sperm DNA fragmentation with intra-cytoplasmic sperm injection clinical outcomes. Cell J 2017;18: 588–96.Google Scholar

Keefe, D, Liu, L, Wang, W, Silva, C. Imaging meiotic spindles by polarization light microscopy: principles and applications to IVF. Reprod Biomed Online 2003;7:24–29.Google Scholar

Fortier, AL, McGraw, S, Lopes, FL et al. Modulation of imprinted gene expression following superovulation. Mol Cell Endocrinol 2014;388: 51–7.CrossRefGoogle ScholarPubMed

Huffman, SR, Pak, Y, Rivera, RM. Superovulation induces alterations in the epigenome of zygotes, and results in differences in gene expression at the blastocyst stage in mice. Mol Reprod Dev 2015;82: 207–17.Google Scholar

Simon, C, Garcia Velasco, JJ, Valbuena, D et al. Increasing uterine receptivity by decreasing estradiol levels during the preimplantation period in high responders with the use of a follicle-stimulating hormone step-down regimen. Fertil Steril 1998;70: 234–39.CrossRefGoogle ScholarPubMed

Wale, PL, Gardner, DK. The effects of chemical and physical factors on mammalian embryo culture and their importance for the practice of assisted human reproduction. Hum Reprod Update 2016;22:2–22.CrossRefGoogle ScholarPubMed

Gardner, DK. Dissection of culture media for embryos: the most important and less important components and characteristics. Reprod Fertil Dev 2008;20:9–18.Google Scholar

Lane, M, Gardner, DK. Understanding cellular disruptions during early embryo development that perturb viability and fetal development. Reprod Fertil Dev 2005;17: 371–78.Google Scholar

Leese, HJ, Biggers, JD, Mroz, EA, Lechene, C. Nucleotides in a single mammalian ovum or preimplantation embryo. Anal Biochem 1984;140: 443–48.Google Scholar

Gardner, DK, Wale, PL. Analysis of metabolism to select viable human embryos for transfer. Fertility and Sterility 2013;99: 1062–72.Google Scholar

Lees, JG, Gardner, DK, Harvey, AJ. Pluripotent stem cell metabolism and mitochondria: beyond ATP. Stem Cells Int 2017;2017:2874283.Google Scholar

Watson, AJ, Kidder, GM, Schultz, GA. How to make a blastocyst. Biochem Cell Biol 1992;70: 849–55.Google Scholar

Warburg, O. On respiratory impairment in cancer cells. Science 1956;124: 269–70.Google Scholar

Gardner, DK, Harvey, AJ. Blastocyst metabolism. Reprod Fertil Dev 2015.Google Scholar

Gardner, DK. Lactate production by the mammalian blastocyst: manipulating the microenvironment for uterine implantation and invasion? Bioessays 2015;37: 364–71.Google Scholar

Bowman, P, McLaren, A. Cleavage rate of mouse embryos in vivo and in vitro. J Embryol Exp Morphol 1970;24: 203–07.Google ScholarPubMed

Quinn, P, Wales, RG. The relationships between the ATP content of preimplantation mouse embryos and their development in vitro during culture. J Reprod Fertil 1973;35: 301–09.Google Scholar

Gardner, DK, Leese, HJ. Concentrations of nutrients in mouse oviduct fluid and their effects on embryo development and metabolism in vitro. J Reprod Fertil 1990;88: 361–68.Google Scholar

Gardner, DK, Sakkas, D. Mouse embryo cleavage, metabolism and viability: role of medium composition. Hum Reprod 1993;8: 288–95.Google Scholar

Lane, M, Gardner, DK. Amino acids and vitamins prevent culture-induced metabolic perturbations and associated loss of viability of mouse blastocysts. Hum Reprod 1998;13: 991–7.Google Scholar

Gardner, DK, Lane, M. Embryo culture systems. In: Gardner, DK, Simon, C, eds. Handbook of In Vitro Fertilization, 4th edition: CRC Press, 2017: 205–44.Google Scholar

Quinn, P, Kerin, JF, Warnes, GM. Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil Steril 1985;44: 493–98.Google Scholar

Carrillo, AJ, Lane, B, Pridman, DD et al. Improved clinical outcomes for in vitro fertilization with delay of embryo transfer from 48 to 72 hours after oocyte retrieval: use of glucose- and phosphate-free media. Fertil Steril 1998;69: 329–34.Google Scholar

Gardner, DK, Lane, M, Calderon, I, Leeton, J. Environment of the preimplantation human embryo in vivo: metabolite analysis of oviduct and uterine fluids and metabolism of cumulus cells. Fertil Steril 1996;65: 349–53.Google Scholar

Gardner, DK, Kelley, RL. Impact of the IVF laboratory environment on human preimplantation embryo phenotype. J Dev Orig Health Dis 2017;8: 418–435.Google Scholar

Leonard, PH, Charlesworth, MC, Benson, L et al. Variability in protein quality used for embryo culture: embryotoxicity of the stabilizer octanoic acid. Fertil Steril 2013;100: 544–49.Google Scholar

Gardner, DK, Rodriegez-Martinez, H, Lane, M. Fetal development after transfer is increased by replacing protein with the glycosaminoglycan hyaluronan for mouse embryo culture and transfer. Hum Reprod 1999;14: 2575–80.Google Scholar

Lawitts, JA, Biggers, JD. Optimization of mouse embryo culture media using simplex methods. J Reprod Fertil 1991;91: 543–56.Google Scholar

Lawitts, JA, Biggers, JD. Culture of preimplantation embryos. Methods Enzymol 1993;225: 153–64.Google Scholar

Ho, Y, Wigglesworth, K, Eppig, JJ, Schultz, RM. Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression. Mol Reprod Dev 1995;41: 232–38.Google Scholar

Gardner, DK, Lane, M. Amino acids and ammonium regulate mouse embryo development in culture. Biol Reprod 1993;48: 377–85.Google Scholar

Biggers, JD, Racowsky, C. The development of fertilized human ova to the blastocyst stage in KSOM(AA) medium: is a two-step protocol necessary? Reprod Biomed Online 2002;5: 133–40.Google Scholar

Morbeck, DE, Baumann, NA, Oglesbee, D. Composition of single-step media used for human embryo culture. Fertil Steril 2017;107:1055–60 e1.Google Scholar

Gardner, DK. Mammalian embryo culture in the absence of serum or somatic cell support. Cell Biol Int 1994;18: 1163–79.Google Scholar

Swain, JE, Lai, D, Takayama, S, Smith, GD. Thinking big by thinking small: application of microfluidic technology to improve ART. Lab Chip 2013;13: 1213–24.Google Scholar

Christianson, MS, Zhao, Y, Shoham, G et al. Embryo catheter loading and embryo culture techniques: results of a worldwide Web-based survey. J Assist Reprod Genet 2014;31: 1029–36.Google Scholar

Kirkegaard, K, Hindkjaer, JJ, Ingerslev, HJ. Effect of oxygen concentration on human embryo development evaluated by time-lapse monitoring. Fertil Steril 2013;99:738–44 e4.Google Scholar

Catt, JW, Henman, M. Toxic effects of oxygen on human embryo development. Hum Reprod 2000;15 Suppl 2:199–206.Google Scholar

Meintjes, M, Chantilis, SJ, Douglas, JD et al. A controlled randomized trial evaluating the effect of lowered incubator oxygen tension on live births in a predominantly blastocyst transfer program. Hum Reprod 2009;24: 300–07.Google Scholar

Gomes Sobrinho, DB, Oliveira, JB, Petersen, CG et al. IVF/ICSI outcomes after culture of human embryos at low oxygen tension: a meta-analysis. Reprod Biol Endocrinol 2011;9:143.Google Scholar

Gardner, DK, Hamilton, R, McCallie, B, Schoolcraft, WB, Katz-Jaffe, MG. Human and mouse embryonic development, metabolism and gene expression are altered by an ammonium gradient in vitro. Reproduction 2013;146:49–61.Google Scholar

Spyropoulou, I, Karamalegos, C, Bolton, VN. A prospective randomized study comparing the outcome of in-vitro fertilization and embryo transfer following culture of human embryos individually or in groups before embryo transfer on day 2. Hum Reprod 1999;14:76–79.Google Scholar

Rijnders, PM, Jansen, CA. Influence of group culture and culture volume on the formation of human blastocysts: a prospective randomized study. Hum Reprod 1999;14: 2333–37.Google Scholar

Almagor, M, Bejar, C, Kafka, I, Yaffe, H. Pregnancy rates after communal growth of preimplantation human embryos in vitro. Fertil Steril 1996;66: 394–97.Google Scholar

Moessner, J, Dodson, WC. The quality of human embryo growth is improved when embryos are cultured in groups rather than separately. Fertil Steril 1995;64: 1034–35.Google Scholar

Ebner, T, Shebl, O, Moser, M et al. Group culture of human zygotes is superior to individual culture in terms of blastulation, implantation and life birth. Reprod Biomed Online 2010;21: 762–68.Google Scholar

Rebollar-Lazaro, I, Matson, P. The culture of human cleavage stage embryos alone or in groups: effect upon blastocyst utilization rates and implantation. Reproductive Biology 2010;10: 227–34.Google Scholar

Kelley, R.L., Gardner, DK. In vitro culture of individual mouse preimplantation embryos: the role of embryo density, microwells, oxygen, timing and conditioned media. Reprod Biomed Online 2017;34: 441–454.Google Scholar

Mortimer, ST, Mortimer, M, Quality and Risk Management in the IVF Laboratory. Cambridge: Cambridge University Press, 2005.Google Scholar

Maas, K, Galkina, E, Thornton, K, Penzias, AS, Sakkas, D. No change in live birthweight of IVF singleton deliveries over an 18-year period despite significant clinical and laboratory changes. Hum Reprod 2016;31: 1987–96.Google Scholar

Hannan, NJ, Paiva, P, Meehan, KL et al. Analysis of fertility-related soluble mediators in human uterine fluid identifies VEGF as a key regulator of embryo implantation. Endocrinology 2011;152: 4948–56.Google Scholar

Truong, TT, Soh, YM, Gardner, DK. Antioxidants improve mouse preimplantation embryo development and viability. Hum Reprod 2016;31: 1445–54.Google Scholar

Hardy, K, Spanos, S. Growth factor expression and function in the human and mouse preimplantation embryo. J Endocrinol. 2002; 172: 221–36.Google Scholar

Robertson, SA, Chin, PY, Schjenken, JE, Thompson, JG. Female tract cytokines and developmental programming in embryos. Adv Exp Med Biol. 2015; 843: 173–213.Google Scholar

Sjoblom, C, Wikland, M, Robertson, SA. Granulocyte-macrophage colony-stimulating factor promotes human blastocyst development in vitro. Hum Reprod. 1999; 14: 3069–76.Google Scholar

Sutton, M, Gilchrist, R, Thompson, J. Effects of in-vivo and in-vitro environments on the metabolism of the cumulus-oocyte complex and its influence on oocyte developmental capacity. Hum Reprod Update 2003; 9: 35–48.Google Scholar

Botigelli, RC, Razza, EM, Pioltine, EM, Nogueira, MFG. New approaches regarding the in vitro maturation of oocytes: manipulating cyclic nucleotides and their partners in crime. JBRA Assist Reprod. 2017; 21: 35–44.Google Scholar

Cavilla, JL, Kennedy, CR, Byskov, AG, Hartshorne, GM. Human immature oocytes grow during culture for IVM. Hum Reprod. 2008; 23: 37–45.Google Scholar

Gilchrist, RB, Thompson, JG. Oocyte maturation: emerging concepts and technologies to improve developmental potential in vitro. Theriogenology. 2007; 1: 6–15.Google Scholar

Mottershead, DG, Sugimura, S, Al-Musawi, SL et al. Cumulin, an oocyte-secreted heterodimer of the transforming growth factor-β family, is a potent activator of granulosa cells and improves oocyte quality. J Biol Chem. 2015; 290: 24007–20.CrossRefGoogle ScholarPubMed

Kaye, PL. Preimplantation growth factor physiology. Rev Reprod. 1997; 2: 121–27.Google Scholar

Harvey, MB, Kaye, PL. Mediation of the actions of insulin and insulin-like growth factor-1 on preimplantation mouse embryos in vitro. Mol Reprod Dev. 1992; 33: 270–75.Google Scholar

Hull, KL, Harvey, S. Growth hormone and reproduction: A review of endocrine and autocrine/paracrine interactions. Int J Endocrinol. 2014; 2014: 1–24.Google Scholar

Kaye, PL, Gardner, HG. Preimplantation access to maternal insulin and albumin increases fetal growth rate in mice. Hum Reprod. 1999; 14: 3052–59.Google Scholar

Lighten, AD, Moore, GE, Winston, RM, Hardy, K. Routine addition of human insulin-like growth factor-I ligand could benefit clinical in-vitro fertilization culture. Hum Reprod. 1998; 13: 3144–50.Google Scholar

Dunglison, GF, Barlow, DH, Sargent, IL. Leukaemia inhibitory factor significantly enhances the blastocyst formation rates of human embryos cultured in serum- free medium. Hum Reprod. 1996; 11: 191–96.Google Scholar

Jurisicova, A, Ben-Chetrit, A, Varmuza, SL, Casper, RF. Recombinant human leukemia inhibitory factor does not enhance in vitro human blastocyst formation. Fertil Steril. 1995; 64: 999–1002.Google Scholar

Brinsden, PR, Alam, V, de Moustier, B, Engrand, P. Recombinant human leukemia inhibitory factor does not improve implantation and pregnancy outcomes after assisted reproductive techniques in women with recurrent unexplained implantation failure. Fertili Steril. 2009; 1: 1445–47.Google Scholar

Kawamura, K, Chen, Y, Shu, Y et al. Promotion of human early embryonic development and blastocyst outgrowth in vitro using autocrine/paracrine growth factors. PLoS ONE. 2012; 7: e49328.Google Scholar

Martin, KL, Barlow, DH, Sargent, IL. Heparin-binding epidermal growth factor significantly improves human blastocyst development and hatching in serum-free medium. Hum Reprod. 1998;13: 1645–52.Google Scholar

O’Neill, C, Ryan, JP, Collier, M et al. Supplementation of in-vitro fertilisation culture medium with platelet activating factor. Lancet. 1989; 2; 769–72.Google Scholar

Sjoblom, C, Wikland, M, Robertson, SA. Granulocyte-macrophage colony-stimulating factor (GM-CSF) acts independently of the beta common subunit of the GM-CSF receptor to prevent inner cell mass apoptosis in human embryos. Biol Reprod. 2002; 67: 1817–23.Google Scholar

Sjoblom, C, Roberts, CT, Wikland, M, Robertson, SA. Granulocyte-macrophage colony-stimulating factor alleviates adverse consequences of embryo culture on fetal growth trajectory and placental morphogenesis. Endocrinology. 2005; 146: 2142–53.Google Scholar

Meintjes, M. Media composition: Macromolecules and embryo growth. In Smith, GD, Swain, JE, Pool, TB (eds.) Embryo Culture: Methods and Protocols, Methods in Molecular Biology. 2012. Springer Science + Business Media, pp. 107–28.Google Scholar

Morbeck, DE, Paczkowski, M, Fredrickson, JR et al. Composition of protein supplements used for human embryo culture. J Assist Genet. 2014; 31: 1703–11.Google Scholar

Bontekoe, S, Johnson, N, Blake, D, Marjoribanks, J. Adherence compounds in embryo transfer media for assisted reproductive technologies: summary of a Cochrane review. Fertil Steril. 2015; 6: 1416–17.Google Scholar

Buck, C, Ruane, P, Smith, H, Brison, D. Effects of hyaluronan-rich culture media on human embryo attachment and gene expression. Proceedings of Fertility 2017, Edinburgh, UK. 2017 P-065: 103.Google Scholar

Cohen, J. (2007): Manipulating embryo development. In: Human Preimplantation Embryo Selection (Eds.: Elder, K. and Cohen, J). Informa Healthcare, Informa UK Ltd,.Google Scholar

Cohen, J., Alikani, M., Trowbridge, J., Rosenwaks, Z. (1992): Implantation enhancement by selective assisted hatching using zona drilling of human embryos with poor prognosis. Hum Reprod 7. 685–91.Google Scholar

Cohen, J., Lindheim, S., Sauer, M. (1999): Assisted hatching causes beneficial effects on the outcome of subsequent frozen embryos transfers of donor oocyte cycle. Fertil Steril 72 (Suppl. 1). S5.Google Scholar

Elhussieny, A., El Mandoub, M., Hanafi, S., Mansour, GM., El-Kotb, A. (2013): Effect of laser assisted hatching on outcome of assisted reproductive technology. Open J Obstet Gynecol 3. 18–23.Google Scholar

The Practice Committee of the Society for Assisted Reproductive Technology and the Practice Committee of the American Society for Reproductive Medicine. (2008): The role of assisted hatching in in vitro fertilization: A review of the literature. A Committee opinion. Fertil Steril 90. S196–98.Google Scholar

The Practice Committee of the Society for Assisted Reproductive Technology and the Practice Committee of the American Society for Reproductive Medicine. (2014): The role of assisted hatching in in vitro fertilization: a guideline. Fertil Steril 102. 348–51.Google Scholar

Cohen, JH., Mather, H., Wright, G. et al. (1989): Partial zona dissection of human oocytes when failure of zona pellucid penetration is anticipated. Hum Reprod 4. 435–42.Google Scholar

Cohen, J., Elsner, C., Kort, H. (1990): Impairment of hatching process following IVF in the human and improvement of implantation by assisted hatching using micromanipulation. Hum Reprod. 5. 7–13.Google Scholar

Rink, K., Delacretaz, G., Salathe, RP. (1996): Non-contact microdrilling of mouse zona pellucida with an objective-delivered 1.48 um diode laser. Laser surg Med 18. 52–62.Google Scholar

Nakayama, T., Fujiwara, H., Yamada, S. et al. (1999): Clinical application of a new assisted hatching method using a piezo-micromanipulator for morphologically low quality embryos: in poor-prognosis infertile patients. Fertil Steril 71. 1014–18.Google Scholar

Cong, F., Tao, L., Ben-Yu, M., Guang-Lun, Z., Canquan, Z. (2010): Mechanically expanding the zona pellucida of human frozen thawed embryos: a new method of assisted hatching. Fertil Steril 94. 1302–1307.Google Scholar

Blake, DA., Forsberg, AS., Johansson, BR., Wikland, M. (2001): Laser zona pellucida thinning-alternative approach to assisted hatching. Hum Reprod 16. 1959–64.Google Scholar

Kanyo, K., Konc, J. (2003): A follow-up study of children born after diode laser assisted hatching. Eur J Obstet Gynecol Reprod Biolo 110. 176–80.Google Scholar

Mansour, RT., Rhodes, CA., Aboulgar, MA., Serour, GI., Kannal, A. (2000): Transfer of zona-free embryos improves outcome in poor prognosis patients: a prospective randomized controlled study. Hum Reprod 15. 161–64.Google Scholar

Carney, SK., Das, S., Blake, D. et al. (2012): Assisted hatching on assisted conception (in vitro fertilisation) (IVF) and intracytoplasmic sperm injection (ICSI). Cochrane Database Syst Rev. 2012 December 12;12:CD001894. doi: 10.1002/14651858.CD001894.pub5.CrossRefGoogle Scholar

Feng, HL., Hershlag, A., Scholl, GM., Cohen, MA. (2009): A retrospective study comparing three different assisted hatching techniques. Fertil Steril 91. 1323–25.Google Scholar

Wellington, PM., Rocha, IA., Ferriani, RA., Nastri, CO. (2011): Assisted hatching of human embryos: a systematic review and meta-analysis of randomized controlled trials. Human Reprod Updates 17. 438–53.Google Scholar

Valojerdi, MR., Eftekhari, YP., Karimian, L., Ashtiani, SK. (2008): Effect of laser zona pellucida opening on clinical outcome of assisted reproduction technology in patients with advanced female age, recurrent implantation failure, or frozen-thawed embryos. Fertil Steril 90. 84–91.Google Scholar

Baruffi, R., Mauri, A., Petersen, C. et al. (2000): Zona thinning with noncontact diode laser in patients aged < 37 years with no previous failure of implantation: a prospective randomized study. J Assist Reprod Genet 17. 557–60.Google Scholar

Frydman, N., Madoux, S., Hesters, L. et al. (2006): A randomized double-blind controlled study on the efficacy of laser zona pellucida thinning on live birth rates in cases of advanced female age. Hum Reprod 21. 2131–35.Google Scholar

Edirisinghe, W., Ahnonkitpanit, V., Promviengchai, S. et al. (1999): A study failing to determine significant benefits from assisted hatching: patients selected for advanced age, zona thickness of embryos, and previous failed attempts. J Assist Reprod Genet 16. 294–301.Google Scholar

Primi, M., Senn, A., Montag, M. et al. (2004): A European multicentre prospective randomized study to assess the use of assisted hatching with a diode laser and the benefit of an immunosuppressive/antibiotic treatment in different patient populations. Hum Reprod 19. 2325–33.Google Scholar

Ng, E., Naveed, F., Lau, R. et al. (2005): A randomized double blind controlled study of the efficacy of laser assisted hatching on implantation and pregnancy rates of frozen–thawed embryo transfer at the cleavage stage. Hum Reprod 20. 979–85.Google Scholar

Kanyo, K., Zeke, J., Kriston, R. et al. (2016): The impact of laser-assisted hatching on the outcome of frozen human embryo transfer cyles. Zygote 24. 42–47.Google Scholar

Ebner, T., Moser, M., Tews, G. (2005): Possible applications of a non-contact 1.48 um wavelength diode laser in assisted reproduction technologies. Hum Reprod Update 11. 425–35.Google Scholar

Zhang, XJ., Yang, YZ., Lv, Q. et al.(2009): Effect of the size of zona pellucida thinning by Laser assisted hatching on clinical outcome of human frozen-thawed embryo transfers. Cryo Letters 30. 455–61.Google Scholar

Hiraoka, K., Hiraoka, K., Horiuchi, T. et al. (2009): Impact of the size of the zona pellucida thinning area on vitrified-warmed cleavage-stage embryo transfers: a prospective, randomized study. J Assist Genet 26. 515–21.Google Scholar