Culture Media, Solutions, and Systems in Human ART

doi:10.1017/CBO9781139059053

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Pramuditha, K. A. S. Hapuarachchi, H. P. Nanayakkara, N. N. Senanayaka, P. R. and De Silva, A.C. 2015. Drawbacks of current IVF incubators and novel minimal embryo stress incubator design. p. 100.

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M. Hozyen, Manar and M. Saadeldin, Islam 2022. Advances in Assisted Reproduction Technologies. Vol. 5, Issue. , p. 153.

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Agarwal, Ashok Maldonado Rosas, Israel Anagnostopoulou, Christina Cannarella, Rossella Boitrelle, Florence Munoz, Lina Villar Finelli, Renata Durairajanayagam, Damayanthi Henkel, Ralf and Saleh, Ramadan 2022. Oxidative Stress and Assisted Reproduction: A Comprehensive Review of Its Pathophysiological Role and Strategies for Optimizing Embryo Culture Environment. Antioxidants, Vol. 11, Issue. 3, p. 477.

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Edited by Patrick Quinn, Sage IVF, Trumbull, Connecticut

Publisher:: Cambridge University Press
Online publication date:: April 2014
Print publication year:: 2014
Online ISBN:: 9781139059053
DOI:: https://doi.org/10.1017/CBO9781139059053

Subjects:: Pathology and Laboratory Science, Obstetrics and Gynecology, Reproductive Medicine, Medicine

59.99 (GBP)

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This volume describes culture media and solutions used in human ART; how they have been developed for in vitro human pre-implantation embryo development, the function and importance of the various components in media and solutions and how they interact, and how the systems in which these are used can influence outcomes. Chapters discuss inorganic solutes, energy substrates, amino acids, macromolecules, cytokines, growth factors, buffers, pH, osmolality, and the interaction of these parameters. The role of incubators and other physical factors are reviewed, along with the relevance and prospects of emerging technologies: morphokinetic analysis using time-lapse imaging and dynamic fluid incubation systems. Results of prospective randomized trials are emphasized to ascertain the added value of these techniques for selecting viable embryos. This comprehensive guide will be invaluable for embryologists, physicians and all personnel involved in the fluid products used in human ART seeking to optimize their successful use of these components.

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Culture Media, Solutions, and Systems in Human ART

This Book has been cited by the following publications. This list is generated based on data provided by Crossref.

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Contents

Culture Media, Solutions, and Systems in Human ART - Half title page
pp i-ii

Culture Media, Solutions, and Systems in Human ART - Title page
pp iii-iii

Copyright page
pp iv-iv

Dedication
pp v-vi

Contents
pp vii-viii

Contributors
pp ix-x

Foreword
pp xi-xi

Section 1 - Culture media and solutions
pp xii-195

Chapter 1 - Overview
pp 1-3

Chapter 2 - Media and embryo interactions
pp 4-20

Chapter 3 - Female tract environment and its relationship to ART media composition
pp 21-29

Chapter 4 - Buffering systems in IVF
pp 30-46

Chapter 5 - Essential features in media development for spermatozoa, oocytes, and embryos
pp 47-67

Chapter 6 - Macromolecular supplementation of embryo culture media
pp 68-79

Chapter 7 - Monozygotic twinning and assisted reproduction. Laboratory or clinical?
pp 80-94

Chapter 8 - Amino acids and ammonium
pp 95-111

Chapter 9 - Growthfactors and cytokines in embryo development
pp 112-131

Chapter 10 - Osmolality
pp 132-141

Chapter 11 - pH control in the embryo culture environment
pp 142-154

Chapter 12 - In vitro maturation of immature human oocytes
pp 155-171

Chapter 13 - Oocyteand embryo cryopreservation media
pp 172-182

Chapter 14 - Internationalregulation of ART media
pp 183-195

Section 2 - Culture systems
pp 196-275

Chapter 15 - Culture of embryos in dynamic fluid environments
pp 197-210

Chapter 16 - Time-lapse imaging of embryo development: using morphokinetic analysis to select viable embryos
pp 211-234

Chapter 17 - Optimizing culture conditions
pp 235-244

Chapter 18 - Low cost IVF
pp 245-257

Chapter 19 - Incubators old and new
pp 258-269

Chapter 20 - Incubator management
pp 270-275

Section 3 - Summary and conclusions
pp 276-278

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Culture Media, Solutions, and Systems in Human ART

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