Book contents
- Principles of IVF Laboratory Practice
- Principles of IVF Laboratory Practice
- Copyright page
- Contents
- Contributors
- Foreword
- The Evolution of IVF Practice
- Section 1 Starting a New Laboratory and Training Protocols
- Section 2 Pre-procedure Protocols
- Section 3 Gametes
- Section 4 Insemination/ICSI
- Section 5 Fertilization Assessment
- Section 6 Embryo Assessment: Morphology and Beyond
- Chapter 29 Polar Body Biopsy for IVF
- Chapter 30 Embryo Assessment at the Pre-compaction Stage in the IVF Laboratory
- Chapter 31 Embryo Assessment at the Post-compaction Stage in the IVF Laboratory
- Chapter 32 Embryo Assessment at the Blastocyst Stage in the IVF Laboratory
- Chapter 33 Trophectoderm Biopsy for Pre-implantation Genetic Testing
- Chapter 34 Embryo Culture by Time-Lapse: Selection and Beyond
- Section 7 Embryo Cryopreservation
- Section 8 Embryo Transfer
- Section 9 Quality Management
- Index
- References
Chapter 30 - Embryo Assessment at the Pre-compaction Stage in the IVF Laboratory
from Section 6 - Embryo Assessment: Morphology and Beyond
Published online by Cambridge University Press: 07 August 2023
- Principles of IVF Laboratory Practice
- Principles of IVF Laboratory Practice
- Copyright page
- Contents
- Contributors
- Foreword
- The Evolution of IVF Practice
- Section 1 Starting a New Laboratory and Training Protocols
- Section 2 Pre-procedure Protocols
- Section 3 Gametes
- Section 4 Insemination/ICSI
- Section 5 Fertilization Assessment
- Section 6 Embryo Assessment: Morphology and Beyond
- Chapter 29 Polar Body Biopsy for IVF
- Chapter 30 Embryo Assessment at the Pre-compaction Stage in the IVF Laboratory
- Chapter 31 Embryo Assessment at the Post-compaction Stage in the IVF Laboratory
- Chapter 32 Embryo Assessment at the Blastocyst Stage in the IVF Laboratory
- Chapter 33 Trophectoderm Biopsy for Pre-implantation Genetic Testing
- Chapter 34 Embryo Culture by Time-Lapse: Selection and Beyond
- Section 7 Embryo Cryopreservation
- Section 8 Embryo Transfer
- Section 9 Quality Management
- Index
- References
Summary
Historically, embryo transfer was performed during the cleavage stage, either on day 2 or day 3. With advancements in embryo culture, many in vitro fertilization (IVF) programmes have moved towards blastocyst transfer. The recent development of pre-implantation genetic screening (PGS), metabolic profiling of conditioned media and time-lapse microscopy provide additional information on embryo selection. However, the assessment of cleavage-stage embryos remains crucial in that the information provides embryologists with a preview of embryo implantation potential. This is especially critical for patients with a limited number of embryos, who do not have the luxury to carry on to blastocyst culture for transfer. At this point, embryo morphology remains the sole selection criterion for choosing embryo(s) for transfer. This chapter discusses embryo assessment with the intention of being a quick guideline for embryo selection/grading with respect to symmetry, multi-nucleation and degree of fragmentation.
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- Principles of IVF Laboratory PracticeLaboratory Set-Up, Training and Daily Operation, pp. 218 - 224Publisher: Cambridge University PressPrint publication year: 2023