In 1987, two applications of the methyl ester of diclofop failed to effectively control Italian ryegrass in some growers' fields. As a result of these observations, studies were initiated to determine if the surviving ryegrass is more tolerant to diclofop than previously untreated ryegrass. Growth chamber studies on five accessions of ryegrass demonstrated that collections from two growers' fields were highly tolerant of diclofop. Diclofop GR50 (rate required to reduce shoot weight by 50%) values of accessions A and B and commercial ryegrass, when diclofop was applied to seedlings at the two-leaf stage, were 2.9, 3.4, and 0.0056 kg ai/ha, respectively. Diclofop-tolerant biotypes were not more tolerant to postemergence barban or preemergence-incorporated triallate plus postemergence diuron or metribuzin. These herbicides are possible alternative treatments in fields where diclofop is no longer effective.