In the Highlands of Madagascar, where rice is the main staple food, explosive demographic growth has driven the need for the development of upland rice. In that context, a Participatory Plant Breeding (PPB) program conducted by the FOFIFA-Cirad partnership, aims to develop upland rice varieties adapted to farmers’ needs, with superior agronomic performances, and with high grain zinc concentration. In the area, where ferralitic soils with N and P deficiencies prevail, limited fertilizer usage persists due to elevated costs, and upland rice varieties must adapt to these low fertility environments. Thus, this paper aims to identify the adequate selection conditions and methods that allow combining the above-mentioned criteria for selection. So, 56 rice breeding lines, including high-zinc genotypes, were evaluated in field trials with contrasting fertility conditions. A relative selection efficiency analysis demonstrated that selection for yield should be done in moderate fertility environments, while selection for grain zinc concentration could be done across a diverse range of conditions. Through participatory evaluations, we identified that, for this case, grain appreciation was the most important character for deciding whether to select a line, followed by productivity and earliness. We also noted that farmers were more willing to accept a variety if it had higher grain zinc concentration. Finally, we proposed a selection index that combines agronomic, farmers’ and nutritional criteria, with the purpose of selecting lines that fulfill the expectations on these areas. Overall, this paper proposes an adapted methodology for the combination of PPB and biofortification in marginal environments.

Ninety-eight high-yielding recombinant inbred lines (RILs) of WAB638-1/PRIMAVERA rice were evaluated with the parents under reproductive stage drought stress. The study aimed at characterizing flowering time response under drought stress and consequently classifying the genetic resources for efficient use in breeding programmes. Two field trials were conducted during the 2016 and 2017 dry seasons using the randomized complete block design with two replications. In 2016, 12 RILs were evaluated with the parents for the rooting attributes under drought stress and well-watered treatments. Analysis of variance revealed a significant (P < 0.05) variation among the genotypes for the traits evaluated. Drought stress reduced most of the characters in this study, including grain yield. However, the inbred lines exhibited considerable tolerance to drought stress as indicated by yield-related stress indexes, including stress susceptibility and drought tolerance indexes. Delayed flowering (FD) was recorded in 75 genotypes (including WAB638-1), while 25 genotypes (including PRIMAVERA) had no delay under drought stress compared to the control. The genotypes were grouped into flowering delay (D) genotypes (FD > 1 d) and no delay (N) genotypes (FD < 1 d), which significantly differ (P ⩽ 0.05) for numbers of days to 50% flowering. The flowering delay genotypes may be suitable for intermittent drought, while genotypes with little or no delay may be selected for terminal drought conditions. There was a significant correlation (r > 0.5) between the root length and number of days to 50% flowering. The study indicated that root parameters, such as the root length, may contribute to the drought adaptation mechanisms of the RILs.

This paper examines genetic erosion in rice landraces thriving in traditional smallholder agricultural systems in the Sarangani uplands, Philippines. In these marginal areas, the crop is closely interwoven with tribal culture and is vital in ensuring food security among upland households. Field visits unveiled high varietal diversity for upland rice and a rich tapestry of indigenous knowledge associated with its cultivation and use. Study results, however, revealed the tapering of the crop's genetic base due to farmers' changing priorities, pest infestation, weakening seed supply systems, shift to cash crops, natural calamities, environmental degradation, government programmes and peace and order problems. Consequently, these pressures undermined traditional agricultural systems in Sarangani upland communities causing food and water scarcity, hunger and suffering on a catastrophic scale. Interdisciplinary strategies aimed at simultaneously averting further varietal losses and environmental degradation while improving human well-being are therefore warranted. Furthermore, making traditional rice farming a lucrative endeavour will induce the younger generation to remain in the uplands and choose farming as a profession. This way, biocultural restoration of agriculture will be attained and the continued presence of the tribal groups in the Sarangani uplands will be ensured for a very long time.

Whether season-long weed control can be achieved in a furrow-irrigated rice system with similar herbicide inputs to that of a flooded system is not known. Field experiments were conducted in 2007 and 2008 at Pine Tree, AR to evaluate different herbicide programs on the weed control efficacy and rice grain yield in furrow-irrigated and flooded rice production systems. Six herbicide programs were evaluated with and without additional late-season “as-needed” herbicide treatments. Minor injury to rice was noted for quinclorac plus propanil. However, the injury was transient and the plants fully recovered. Overall weed control was greater in the flooded system compared with the furrow-irrigated system (up to 20% greater), because flooding effectively prevented the emergence of most terrestrial weeds. In addition, rice grain yields were 13 to 14% greater in flooded compared with furrow-irrigated plots. Irrespective of the irrigation system, herbicide programs that contained a PRE-applied herbicide provided greater weed control and resulted in greater yield compared with those that did not contain PRE-applied herbicide, indicative of the importance of early-season weed control in achieving higher grain yields. On the basis of weed control, yield, and weed treatment cost, the herbicide program with clomazone PRE followed by propanil at four- to five-leaf rice was more efficient than other programs evaluated in both irrigation systems. However, furrow-irrigated plots required as-needed herbicide applications, which were applied after the four- to five-leaf rice stage when two or more plots within a program exhibited ≤ 80% control for any of the weed species. This suggests that furrow-irrigated rice production demands additional weed management efforts and thereby increases production costs. There is also a possibility for substantial yield reduction in the furrow-irrigated system compared with the flooded system. Nevertheless, furrow-irrigated rice production can still be a viable option under water-limiting situations and under certain topographic conditions.

Local upland rice germplasm is an invaluable resource for farmers who grow rice on acidic soils without flooding that benefits wetland rice. In this study, we evaluated the adaptation to soil acidity in common local upland rice varieties from an area with acidic soil in Thailand. Tolerance to hydrogen and aluminium (Al) toxicity was determined by measuring root growth, plant dry weight and phosphorus (P) uptake in aerated solution culture without the supplementation of Al (0 mg/l) at pH 7 and 4 and with the supplementation of 10, 20 and 30 mg Al/l at pH 4. The root growth of upland rice plants grown from farmers' seed was depressed less by Al than that of common wetland rice varieties. Pure-line genotypes of upland rice varieties were differentiated into several classes of Al tolerance, with frequency distribution of the classes that sometimes differed between the accessions of the same varieties. The effect of Al tolerance on root length was closely correlated with depression by Al in root dry weight and whole-plant P content. A source for adaptation to soil acidity for exploitation in the genetic improvement of aerobic and rainfed rice is clearly found among local upland rice varieties grown on acidic soils. However, the variation in tolerance to soil acidity within and among the seed lots of the same varieties maintained by individual farmers as well as among the varieties needs to be taken into consideration.

This study examined variation in seed zinc (Zn) in a local upland rice germplasm that may affect adaptation in a system of slash-and-burn. Individual seed Zn in farmers' seed lots of local upland rice varieties from a slash-and-burn system was evaluated by staining with dithizone (DTZ). Concentration of Zn in the farmers' seed lots and their single-seed descent genotypes grown at Chiang Mai University was determined by chemical analysis. The DTZ staining of individual seeds in most of the farmers' seed lots covered the intensity of standards that ranged from 19 mg Zn/kg (RD21) to 31 (Nam Roo) mg Zn/kg. Zinc content by chemical analysis was closely correlated with the weighted-average staining for each seed lot. Almost all of the single-seed descent genotypes had higher seed Zn than RD21; two-thirds were higher than Nam Roo. The variation within seed lots detected by DTZ staining was confirmed by seed Zn in the single-seed descent genotypes. Evolutionary adaptation to soil with limited Zn, exacerbated by alkalinity of the ash from slash-and-burn, is made possible by variation in seed Zn among individuals growing together in the same field, and benefits the eaters with Zn-enriched seed.

The development of near-isogenic lines (NILs) is an important fundamental step in the cloning of quantitative trait loci (QTL) and molecular marker assisted breeding. In the present study, NILs for two major QTLs of basal root thickness (BRT) and 1000-grain-weight (TGW) were obtained by molecular marker-assisted selection (MAS) through foreground selection for target QTL and background selection in three backcross generations (BC1F1, BC2F1 and BC3F1). After phenotypic evaluation in the BC3F2 generation, nine BRT QTL-NILs with BRT ranges of 1.07–1.16 mm (6.11–15.18% greater than the recurrent parent) and an average recovery ratio of genetic background (RRGB) of 97.22%, and 11 TGW QTL-NILs with ranges of 21.25–26.25 g (7.05–32.16% greater than the recurrent parent) and 95.97% of RRGB, were selected.

Studies were carried out in 1983 to determine the relative abundance and species composition of three lepidopterous stem borers in upland and irrigated rice at Ibadan, southwestern Nigeria. The relationship between irrigated rice crop phenology and the population of the borers and stalk-eyed fly was also studied.

White stem borer, Maliarpha separatella was the predominant species in upland and irrigated rice, followed in upland rice by pink stem borer, Sesamia calamistis, and striped stem borer, Chilo zacconius and in irrigated rice by C. zacconius and S. calamistis. The proportion of M. separatella was higher in irrigated rice than upland. Three population peaks were observed annually in both upland and irrigated rice.

Studies on the relationship between irrigated rice phenology and field population of lepidopterous borers and a dipteran borer showed that the stalk-eyed fly, Diopsis spp., occurred before 20 days after transplanting (DAT) in ITA 212 and continued up to 90 DAT: the peak was observed at 40 DAT. The lepidopterous borers did not appear until 40 DAT and populations peaked at 80 DAT.

Stem-borer incidence on upland and irrigated rice varieties is compared under natural or artificial infestation, and with simulated damage by sectioning tillers. In the early stages, Diopsids lay more eggs on irrigated varieties, but these are less suitable for the development of Diopsids larvae than upland varieties, whose stems are thicker.

Some varieties of Oryzae glaberrima show a high level of resistance to stem-borers and Hispids. Little difference is noticed for antibiosis of the Oryzae sativa studied, and losses depend on the tolerance of varieties. Irrigated varieties have a better tolerance because of their high tillering, preventing the attack, and of their ability to compensate for early losses by producing new tillers. This compensation does not exist for upland varieties. Their tolerance depends on tillering rate, the ability of infested stems to produce fertile panicles, and the difference in productivity between primary and secondary tillers, the former being more frequently infested. For the same population of stem-borers, losses observed on upland rice are three times lower on tolerant varieties than non-tolerant ones.