We documented the existence of a population of the southern Caribbean pontarachnid mite Litarachna caribica for the first time on the Pacific coast of Panama. Based on morphological observations, this is the first record of a pontarachnid mite with a trans-isthmian distribution, which can be explained by either modern biological dispersal or historical vicariance hypotheses. Litarachna caribica had either passed through the Panama Canal, successfully colonizing the opposite coast, or previously continuously distributed populations had become disjunct after the rise of the Central American land.