A technique for infecting adult tsetse with an entomopathogenic fungus was developed and tested in the laboratory. A nitrocellulose filter membrane (Millipore) was used as substrate for retaining conidia after fitration, and a cylindrical plastic tube served as a chamber for the flies. The technique was designed to allow accurate estimation of inoculum on the substrate, thereby permitting comparison of virulence during the screening of fungal pathogens against tsetse flies. Glossina morsitans centralis adults were susceptible to Metarhizium anisopliae at all doses tested, and mortalities were dose dependent. No significant differences in mortalities were observed when flies were exposed for different durations to the pathogen. The number of conidia picked up by flies varied considerably with exposure time and concentration.
