Little information is available regarding the calcium (Ca) dynamics and how its concentration is influenced following the Ca treatment (injection or bolus) after calving in dairy cows. To evaluate the short- and long-term effects of different sources of Ca supplement to animals fed anionic diets during the pre-partum period, 36 multiparous Holstein cows were randomly assigned to 1 of 3 treatments: (1) control group without Ca supplement (CON); (2) subcutaneous injection of 500 ml of 40% w/v Ca borogluconate immediately post-calving (SUB) and (3) oral supplement of Ca bolus containing 45 g Ca immediately and 24 h post-calving (BOL). Serum concentrations of Ca, P and Mg were measured. Serum concentration of Ca was affected by treatments at 48 h of post-calving (P < 0.01). The mean Ca at 6 h was greater in SUB compared to CON group (2.34 v. 2.01 mmol/l; P < 0.002). The lowest Ca concentration at 12 h was related to CON cows compared with BOL and SUB cows (1.90, 2.16 and 2.14 mmol/l, respectively; P < 0.02); a similar trend was observed 24 h post-calving (P < 0.02). Serum concentrations of P and Mg were not influenced by treatments. Yield of milk, milk protein and fat-corrected milk were lowest (P < 0.05) in SUB cows within 3 weeks of lactation in comparison with CON and BOL cows. However, milk yield and milk composition did not show any difference among treatments throughout the first 3 months post-calving. In general, under conditions of this experiment, Ca supplements to fresh cows as an oral bolus are recommended in comparison with subcutaneous injection.