Sub-annual measurements, eight increments per year, of cellulose in a Scots pine tree growing in central Norway are presented as a proxy for tropospheric 14CO2 at biweekly to monthly resolution. The results are validated by comparison to direct atmospheric measurements in the years 1959–1965, and a new dataset is obtained for 1953–1958. In this period, our cellulose measurements deviate from the Bomb 13 NH1 calibration curve, which is derived from single-year measurements of tree rings. This is due to seasonal cycles in tropospheric radiocarbon (14C) concentrations, caused by the first series of atmospheric nuclear weapons tests.