A 2-yr field study was conducted to compare growth characteristics of 24 Palmer amaranth accessions collected from across the indigenous range of the species in the United States. Variation in growth and development of Palmer amaranth was noted among accessions based on leaf area ratio (LAR), specific leaf area (SLA), net assimilation rate (NAR), and stem leaf ratio (SLR), but only SLR varied across harvest intervals among accessions. Accessions collected across the range of Palmer amaranth in the United States displayed variation in growth and development based on differences in LAR, SLA, NAR, and SLR. Observed differences among accessions indicate the existence of Palmer amaranth ecotypes.