Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
  1. Home
  2. Search

Refine search

Refine search

Access:
Content type:
Publication date:
Apply   From and To filters
Subject: Show more
Journals Show more
Publishers: Show more
Societies Show more
Series: Show more
Collections: Show more

Actions for selected content:

Select all | Deselect all
  • View selected items
  • Export citations
  • Download PDF (zip)
  • Save to Kindle
  • Save to Dropbox
  • Save to Google Drive

Save Search

You can save your searches here and later view and run them again in "My saved searches".

Please provide a title, maximum of 40 characters.
Cancel
×

1 results

  • Chapter 13 - Future Directives in Sperm Handling for ART

  • from Part III - Laboratory Handling of Retrieved Sperm
  • Edited by Ashok Agarwal, Ahmad Majzoub, Sandro C. Esteves
  • Book:
    Manual of Sperm Retrieval and Preparation in Human Assisted Reproduction
    Published online:
    09 April 2021
    Print publication:
    10 June 2021, pp 117-130

  • Summary

    Couple infertility is gradually increasing, and couples unable to conceive naturally are dependent on assisted reproductive techniques (ART). Intracytoplasmic sperm injection (ICSI) bypasses the natural selection process of sperm in the female reproductive tract, hence sperm-handling techniques are used to select the most suitable sperm for oocyte fertilization. In this chapter we discuss the conventional sperm-processing methods such as swim-up and density gradient centrifugation. Furthermore, advanced techniques such as magnetic-activated cell sorting, microfluidic devices, motile sperm organelle morphology examination, flow cytometry, and zeta potential are presented. We provide an overview of the sperm-handling approach and its outcome in ART. Additionally, we highlight the future sperm-handling techniques such as Raman spectrometry, interferometric phase microscopy, confocal light absorption and scattering spectroscopy, proteomic analysis, and peptide-based selection of sperm.