The distribution of glycine-immunoreactive (glycine-IR) neurons
and their
associated axon terminals in the
rat cuneate nucleus was studied using antiglycine postembedding immunoperoxidase
labelling and
immunogold staining, respectively. The immunoperoxidase-labelled glycine-IR
neurons were widely
distributed in the entire rostrocaudal extent of the nucleus. They made
up 30.8%
(9671/31368) of the
neurons surveyed. Quantitative evaluation showed that the percentage of
glycine-IR neurons in the caudal
level was significantly higher than that in the middle and rostral levels.
The
glycine-IR neurons were small
cells (mean area=198±1.9 μm2, n=2862) with ovoid or
spindle-shaped somata. Statistical analysis showed
that the size of the glycine-IR neurons in the rostral level was significantly
smaller than that in the middle
and caudal levels. Immunogold labelled glycine-IR terminals which contained
predominantly pleomorphic
synaptic vesicles were mostly small (mean area=1.24±0.03 μm2,
n=286) and they constituted 24.7%
(286/1158) of the total terminals surveyed. They formed axodendritic,
axosomatic and axoaxonic synapses
with unlabelled elements. It is suggested from this study that glycine
is one
of the major neurotransmitters
involved in the depression of synaptic transmission in the cuneate nucleus.