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Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins
SUSAN K. LYMAN, LARRY GERACE, SUSAN J. BASERGA
Journal:

RNA / Volume 5 / Issue 12 / December 1999

Published online by Cambridge University Press:

28 August 2001, pp. 1597-1604

Print publication:

December 1999
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We have identified an apparent human homolog of the yeast Nop5/Nop58 protein. hNop5/Nop58 codes for a protein of predicted molecular weight 59.6 kDa and is 46.8% identical to Saccharomyces cerevisiae Nop5/Nop58. Immunofluorescent staining with antibodies against hNop5/Nop58 indicate that it is localized primarily to the nucleolus, and coimmunoprecipitation from nuclear extracts demonstrates that hNop5/Nop58 interacts with the box C/D family of snoRNAs. Thus, hNop5/Nop58 is a common component of the box C/D snoRNPs, and joins fibrillarin as the second such component identified and characterized in metazoans.