A study of the resting site preferences of Glossina palpalis gambiensis in an area of a gallery forest in the Northern Guinea Savanna of Mali, infested exclusively by G. p. gambiensis was conducted in February, 1980 (hot dry season). Five site-types were compared: fallen logs, boles, undergrowth, tree canopies and branches. Distribution of resting flies was as follows: fallen logs (55.62%), boles (35.24%), undergrowth (8.4%), tree canopies (0.61%) and branches (0.00%). Most of the flies were collected during the periods 06.00–09.00 (37.47%) and 09.00–12.00 hr (46.5%). On boles, 86.06% of flies were found below 1.0 m and no flies were collected above 2.0 m. These results are compared to those obtained during simultaneous studies on the resting site preferences of G. p. gambiensis in an area along the same gallery forest, with mixed G. p. gambiensis and G. morsitans submorsitans infestation. Possible reasons for the differences observed are discussed.