Five genera and eight species of gastropods of families Conidae and Conilithidae were observed in their natural habitats on the southernmost portion of the Mesoamerican Barrier Reef, off the northern coast of Roatan Island, Honduras. Fifty per cent of species are widespread Caribbean–western Atlantic species, whereas 50% are endemic to the Nicaraguan biogeographical subprovince and Roatan Island. Multiple sightings during night SCUBA diving operations revealed that the reef off northern Roatan supports a healthy and diverse population of conoidean gastropods. Distribution of all recorded species by depth and habitat type revealed a distinct reef partitioning between the 4 most commonly occurring species.