We report three reading comprehension experiments investigating the interpretational preferences and processing of pro and overt pronouns in Chinese, a ‘discourse-oriented’ pro-drop language (Huang 1984). Our offline rating experiments showed that both pro and overt pronouns were subject-based, but the preference for the subject antecedents was stronger with pro than with overt pronouns. In addition, these different levels of subject biases were confirmed in a self-paced reading experiment; a processing penalty was incurred with object antecedent interpretation regardless of the pronominal type, but the penalty was bigger for pro than for overt pronouns. These experimental results are consistent with Accessibility theory that less specific anaphoric expressions (e.g. pro) were less likely than more specific anaphoric expressions (e.g. overt pronouns) to refer to a less prominent antecedent (e.g. syntactic object).