Radiation therapy, widely used in the treatment of a variety of malignancies in the pelvic area, is associated with inevitable damage to the surrounding healthy tissues. We have applied atomic force microscopy (AFM) to track the early damaging effects of ionizing radiation on the collagen structures in the experimental animals’ bladder and rectum. The first signs of the low-dose radiation (2 Gy) effect were detected by AFM as early as 1 week postirradiation. The observed changes were consistent with initial radiation destruction of the protein matrix. The alterations in the collagen fibers’ packing 1 month postirradiation were indicative of the onset of fibrotic processes. The destructive effect of higher radiation doses was probed 1 day posttreatment. The severity of the radiation damage was proportional to the dose, from relatively minor changes in the collagen packing at 8 Gy to the growing collagen matrix destruction at higher doses and complete three-dimensional collagen network restructuring towards fibrotic-type architecture at the dose of 22 Gy. The AFM study appeared superior to the optical microscopy-based studies in its sensitivity to early radiation damage of tissues, providing valuable additional information on the onset and development of the collagen matrix destruction and remodeling.

Bumblebees need to keep bodily homeostasis and for that have an efficient system of excretion formed by the Malpighian tubules, ileum, and rectum. We analyzed the excretory organs of Bombus morio, a bee without rectal pads. In addition, we analyzed the rectal epithelium of Melipona quadrifasciata anthidioides which has rectal pads. The Malpighian tubules exhibited two cell types and the ileum four types. However, comparative analysis of the rectum showed that only cells of the anterior region of the rectal epithelium of B. morio are structurally distinct. We suggest that cells of the Malpighian tubules of B. morio have an excretory feature and that cells of ileum have different functions, such as ion absorption and water, organic compound, and protein secretion. In addition, only the anterior region of the rectum of B. morio showed characteristic absorption. We suggest that Malpighian tubules participate in the excretion of solutes and that the ileum and rectal epithelium are responsible for homeostasis of water and solutes, compensating for the absence of rectal papillae. These results contribute to our understanding of the morphophysiology of the excretory organs of bees without rectal pads.

Purpose: The organ at risk in prostate radiotherapy is the anterior rectal wall . This pilot study was conducted to quantify the inter-observer variability of rectal contouring in conformal prostate radiotherapy planning using four contouring methods and to determine a standard rectal contouring definition.

Methods and materials: Ten patients with T1/T2 disease and six clinical oncologists were recruited. Two cross-sectional and two length limits generated four rectal volumes. Each clinician contoured the four volumes for each patient and the dose–volume histograms (DVHs) were analysed. The percentage rectal volume receiving 20%, 50%, 80%, 90% and 95% of the total delivered dose and the mean and median rectal doses were calculated. Data were presented as mean ± 2 standard deviations.

Results: The Sh Rec method (contouring the rectum including its contents extending from 2 cm above the upper limit of the prostate to 2 cm below the prostatic apex) was the least variable in the 80%, 90% and 95% percentage ranges. The mean difference in Sh Rec-contoured volume was 18.7 cm3 (± 22.3 cm3).

Conclusions: The Sh Rec-contouring method showed the least inter-observer variability. The results are informative and will help define a standard rectal contouring method.