Microvibrations originating from onboard disturbance sources can lead to a range of issues, including a decrease in satellite pointing accuracy, image distortion and blurring. Therefore, reaction wheels emerge as the primary sources of disturbance noise. This paper employs an experimental approach based on the real dynamics of rotating reaction wheel assembly, closely simulating on-orbit configurations to measure noise responses transferred to the satellite structure. An assessment of noise response behaviour, incorporating a comprehensive understanding of the factors influencing the levels, was conducted on a proto-flight satellite for three reaction wheels. Initially, reaction wheel assemblies underwent individual iterative balancing to reduce mass deviations. Subsequently, amplitude-time responses at different rotational speeds of reaction wheel assemblies (RWA) disturbance noise were measured. The experimental results demonstrate that each individually balanced reaction wheel generates independent perturbation noise level due to manufacturing imperfections. Hence, the necessity of wheels testing for accurate prediction and mitigation of disturbance levels is crucial, especially for payloads sensitive to microvibrations. Furthermore, increasing wheel speeds proportionally amplify disturbance noise levels. Therefore, implementing an optimised mission attitude control profile with lower rotation speeds of reaction wheels effectively reduces microvibration levels which minimises risks to payload performance and reduce power consumption.